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Sequencing of human HFF fibroblasts during replicative senescence (smallRNA)

ABSTRACT: Sequencing of human HFF fibroblasts during replicative senescence (smallRNA)

PROVIDER: PRJNA483225 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7613080.fastq.gz	Fastqsanger.gz
	SRR7613081.fastq.gz	Fastqsanger.gz
	SRR7613072.fastq.gz	Fastqsanger.gz
	SRR7613082.fastq.gz	Fastqsanger.gz
	SRR7613073.fastq.gz	Fastqsanger.gz

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Sequencing of human HFF fibroblasts during replicative senescence (smallRNA)

Project description:small RNA gene expression profiles of senescent human fibroblasts were compared to young proliferating fibroblasts. RNA-seq data comprises 5 groups: 16, 26, 46, 64 and 74 population doublings of HFF fibroblasts. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)

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Project description:Telomere shortening in populations of human mammary epithelial cells (HMECs) that survive early replicative arrest (M0) by the inactivation of p16INK4A during cell culture on plastic dishes leads to a state of permanent replicative arrest termed senescence. While culture of HMECs on feeder layers abrogates M0 and p16INK4A inactivation, progressive telomere attrition in these cells also eventually results in permanent replicative arrest. Expression of telomerase prevents both senescence on plastic (S-P) and senescence on feeder layers (S-FL) in HMECs, as it does also in cultured primary human fibroblasts. We report here that the gene expression profiles of senescence in HMECs of the same lineage maintained under different culture conditions showed surprisingly little commonality. Moreover, neither of these senescence-associated profiles in HMECs resembles the profile for senescence in human fibroblasts. These results indicate that senescence-associated alterations in gene expression resulting from telomere attrition are affected by culture conditions as well as by cell origins, and argue that replicative senescence at the molecular level is a diverse rather than unique cellular process.

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Project description:By transcriptome analysis of IMR-90 human fibroblasts following oncogene-induced senescence (OIS) and replicative senescence (RS), we identified commonly regulated genes in both conditions.

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Human primary fetal lung fibroblasts (HFL-1) undergoing replicative senescence

Project description:Human primary fetal lung fibroblasts (HFL-1) undergoing replicative senescence

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Transcription profiling of human mammary epithelial cells HMECs (S-FL, S-P and PGA) during replicative senescence

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Project description:This SuperSeries is composed of the following subset Series: GSE3730: Replicative senescence in human fibroblasts GSE3731: Replicative senescence in post-selection HMECs Abstract: Replicative senescence is the state of irreversible proliferative arrest that occurs as a concomitant of progressive telomere shortening. By using cDNA microarrays and the gabriel system of computer programs to apply domain-specific and procedural knowledge for data analysis, we investigated global changes in gene transcription occurring during replicative senescence in human fibroblasts and mammary epithelial cells (HMECs). Here we report the identification of transcriptional "fingerprints" unique to senescence, the finding that gene expression perturbations during senescence differ greatly in fibroblasts and HMECs, and the discovery that despite the disparate nature of the chromosomal loci affected by senescence in fibroblasts and HMECs, the up-regulated loci in both types of cells show physical clustering. This clustering, which contrasts with the random distribution of genes down-regulated during senescence or up-regulated during reversible proliferative arrest (i.e., quiescence), supports the view that replicative senescence is associated with alteration of chromatin structure. Refer to individual Series

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