Project description:To examine the role of NONO in estrogen-independent breast cancer, MDA-MB-231 cells were treated with siRNA targeting NONO or control siRNA (siControl). Microarray analysis revealed NONO-regulated genes in MDA-MB-231 cells.
Project description:To examine the role of PSF and NONO in estrogen-dependent breast cancer, MCF-7 cells were treated with siRNA targeting PSF, NONO or control siRNA (siControl). Microarray analysis revealed PSF- or NONO-regulated genes in MCF-7 cells.
Project description:To examine the role of PSF and NONO in breast cancer cells, PSF- and NONO- associated transcripts were co-immunoprecipitated with PSF and NONO antibodies, respectively. Purified RNAs were analyzed with NGS.
Project description:We used single cell RNA sequencing (scRNA-seq) to analyze the diversity and gene expression of testis cells in the adult NONO and NONO△ER mice testis