Gene expression analysis of neutrophils, Ym1+Ly6Chi monocytes and Ym1-Ly6Chi monocytes
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA484126 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR7637740.fastq.gz | Fastqsanger.gz | |||
| SRR7637741.fastq.gz | Fastqsanger.gz | |||
| SRR7637742.fastq.gz | Fastqsanger.gz | |||
| SRR7637743.fastq.gz | Fastqsanger.gz | |||
| SRR7637739.fastq.gz | Fastqsanger.gz |
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Project description:Analysis of Ly6Chi monocytes from small intestine lamina propria (SILP) and blood of day 8 Toxoplasma gondii infected mice at gene expression level. The hypothesis tested in the present study was that Ly6Chi monocytes from SILP have altered expression of regulatory factors to blood monocytes. Results provide important information on the regulatory to effector balance of genes expressed by Ly6Chi monocytes during an acute inflammatory response. Ly6Chi inflammatory monocytes were sorted by FACS from the blood or small intestine lamina propria (SILP) of Toxoplasma gondii infected C57BL/6 mice. Cells were isolated at day 8 after infection and total RNA obtained from sorted populations. Three biological replicates were acquired for both blood and SILP from pooled animals.
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