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Next Generation Sequencing Facilitates Quantitative Analysis of Normoxia/Hypoxia Human Dental Pulp Cells Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Normoxia/Hypoxia Human Dental Pulp Cells Transcriptomes

PROVIDER: PRJNA484181 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7638360_1.fastq.gz	Fastqsanger.gz
	SRR7638360_2.fastq.gz	Fastqsanger.gz
	SRR7638370_1.fastq.gz	Fastqsanger.gz
	SRR7638370_2.fastq.gz	Fastqsanger.gz
	SRR7638361_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of Normoxia/Hypoxia Human Dental Pulp Cells Transcriptomes

Project description:We report the application of high-throughput profiling of gene expression in human dental pulp cells under normoxia and hypoxia conditions.

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Project description:Hypoxia enhances the reprogramming efficiency of human dermal fibroblasts to become induced pluripotent stem cells (iPSCs). Because we showed previously that the hypoxia facilitates the isolation and maintenance of human dental pulp cells (DPCs), we examined here whether it promotes the reprogramming of DPCs to become iPSCs. To investigate the effect of oxygen concentration on global gene expression, we compared DPCs cultured for 6 days under hypoxia and normoxia.

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SAHA Rat Dental Pulp

Project description:Transcriptional response of rat dental pulp cells (DPCs) cultured with SAHA at early and late mineralisation time points Transcript profiling of DPC identified several novel genes expression induced and supressed by HDACi at 24 hrs and 14 days under mineralising conditions. SAHA induces several members of the MMP family of endopepsidases (TIMP-1, MMP-9, MMP-13) and other members of the endochondral ossification pathway at 24 h. 8 experiemental parameters were analysed, each carried out in quadruplicate

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Project description:Wnt regulates various cell responses. In dental pulp cells, Wnt signaling control cell proliferation, apoptosis, migration and differentiation. Here, the differential gene expression of human dental pulp stem cells treated with Wnt ligands or Wnt agonist was examined using a high throughput RNA sequencing technique. Results demonstrated that Wnt ligands or Wnt agonist altered numerous gene expression in human dental pulp stem cells.

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