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RNA-sequencing of mouse double-negative developing thymocytes [WT and Duxbl[ind]xpTa[Cre]]

ABSTRACT: RNA-sequencing of mouse double-negative developing thymocytes [WT and Duxbl[ind]xpTa[Cre]]

PROVIDER: PRJNA484208 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7639460.fastq.gz	Fastqsanger.gz
	SRR7639461.fastq.gz	Fastqsanger.gz
	SRR7639462.fastq.gz	Fastqsanger.gz
	SRR7639463.fastq.gz	Fastqsanger.gz
	SRR7639456.fastq.gz	Fastqsanger.gz

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RNA-sequencing of mouse double-negative developing thymocytes [WT and Duxbl[ind]xpTa[Cre]]

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RNA-sequencing of mouse double-negative developing thymocytes

Project description:RNA-sequencing of mouse double-negative developing thymocytes

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Project description:rs10-03_ind - comparison of ind induced vs mock treated seedlings - Identification of miRNAs regulated by IND - Transgenic seeds containing an IND transgene under the control of a dexamethasone inducible promoter were germinated and grown in liquid Murashige/Skoog medium under a 16h light/8h darkness light regime. After seven days of growth, seedlings were either mock-treated or treated with 10 micromolar dexamethasone for 24 hours.

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Project description:Upon exit from the totipotent 2-cell (2C) embryo stage, the 2C-associated transcriptional program needs to be efficiently silenced. However, the molecular mechanisms involved in this process remain mostly unknown. Here, we determined that the 2C-specific transcription factor DUX directly induced the expression of DUXBL to promote this silencing. CUT&RUN analysis revealed that DUXBL gained accessibility to DUX-bound regions in DUX-induced ESC while it was unable to bind those regions in uninduced cells. Importantly, DUX expression in Duxbl-knockout ESC caused increased induction of the 2C-transcriptional program, whereas DUXBL overexpression impaired 2C-associated transcription. Mechanistically, we determined that DUXBL interacted with TRIM24 and TRIM33, two members of the tripartite motif superfamily involved in gene silencing, and co-localized with them in nuclear condensates upon DUX expression. Importantly, DUXBL downregulation in mouse zygotes led to a penetrant 2C-stage arrest. Our data revealed an unexpected role for DUXBL in controlling the exit from totipotency.

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