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Identification of Transcription Factor Binding Sites using ATAC-seq

ABSTRACT: Identification of Transcription Factor Binding Sites using ATAC-seq

PROVIDER: PRJNA484892 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7652720_1.fastq.gz	Fastqsanger.gz
	SRR7652720_2.fastq.gz	Fastqsanger.gz
	SRR7652721_1.fastq.gz	Fastqsanger.gz
	SRR7652721_2.fastq.gz	Fastqsanger.gz
	SRR7652718_1.fastq.gz	Fastqsanger.gz

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Identification of Transcription Factor Binding Sites using ATAC-seq

Project description:Dendritic cells (DC) are professional antigen presenting cells that comprise different subsets: classical DC type 1 and 2 (cDC1 and cDC2, respectively) and plasmacytoid DC (pDC). In this study cDC1 or pDC were obtained in a two-step in vitro culture system according to Felker et al., J. Immunol. 185, 5326-5335, 2010. Briefly, mouse bone marrow cells were first amplified with a specific cytokine cocktail and then induced to differentiate into DC with Flt3 ligand. cDC1 are CD11c+ CD11b+ XCR1+ and pDC are CD11c+ CD11b- B220+ and thus cDC1 and pDC were obtained by FACS sorting and subjected to Omin-ATAC-seq analysis. As an example of practical application of HINT-ATAC, we performed Omin-ATAC-seq experiments of cDC and pDC cell populations and used HINT-ATAC to detect footprints within ATAC-seq peaks of each of these two cells. Next, we estimated changes in binding activity for all factors with a motif in JASPAR. Cell-specific TF activity is measured by the depth of footprints and number of reads flanking regions. Two known cDC factors, BATF3 and HES1, are detected. In summary, we demonstrated that HINT-ATAC is capable of identifying relevant factors for dendritic cell specification.

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