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The role of cardiomyocyte Muscle Ring Finger-1 (MuRF1) in transcriptional responses to cyclic stretch (Wei HL1 str RNA seq oridata 71917)

ABSTRACT: The role of cardiomyocyte Muscle Ring Finger-1 (MuRF1) in transcriptional responses to cyclic stretch (Wei HL1 str RNA seq oridata 71917)

PROVIDER: PRJNA484903 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7652880_1.fastq.gz	Fastqsanger.gz
	SRR7652880_2.fastq.gz	Fastqsanger.gz
	SRR7652890_1.fastq.gz	Fastqsanger.gz
	SRR7652890_2.fastq.gz	Fastqsanger.gz
	SRR7652900_1.fastq.gz	Fastqsanger.gz

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The role of cardiomyocyte Muscle Ring Finger-1 (MuRF1) in transcriptional responses to cyclic stretch (Wei HL1 str RNA seq oridata 71917)

Project description:We investigated the role of cardiomyocyte MuRF1 in the transcriptional responses to cyclic stretch (15% biaxial stretch at 1 Hz for 60 min) using the HL-1 cell line.

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Phosphoproteome of cyclic stretch A549 and IMR-90

Project description:Lung cancer is one of the leading causes of death. However, most of the researches were based on the traditional cell-culturing method. Whereas cells of lung are subjected to the mechanical forces periodically while breathing. In the present study, we applied cyclic stretch to stimulate the continuously contracting physical condition. We uncovered the stretching force-induced phosphoproteome in lung cancer cell A549 and fibroblast IMR-90. 2048 and 2604 phosphosites corresponding to 837 and 1008 phosphoproteins were identified in A549 and IMR-90, respectively. Interestingly, cytoskeleton reorganization and mitochondrial localization were enriched in the significantly expressed phosphoproteins in response to cyclic stretch. Indeed, we found this physical stress changed cell alignment thus disrupted mitochondrial dynamics. We proved that mitochondrial fusion is induced by uniaxial stretch in 2 cell lines. This study reveals the molecular mechanism of cyclic stretch and supports that stretching force enhanced cellular rearrangement and mitochondrial fusion in lung cells.

2021-09-08 | PXD012010 | Pride

Transcription profiling of human bladder smooth muscle cells subjected to cyclic stretch-relaxation

Project description:The aim of the experiment was to determine the effect of cyclic stretch-relaxation ("stretch") on gene expression patterns in normal diploid human bladder smooth muscle cells. Cells plated on silicone elastomer bottomed 6-well culture dishes were grown to ~80% confluence, serum-depleted for 48h and subjected to cyclic stretch-relaxation at 20% elongation for 4h. Cells seeded in stretch plates but not subjected to stretch served as controls. Total RNA was extracted from both groups of cells, reverse-transcribed, biotin-labeled, fragmented and hybridized to HG-U133A. Four biological replicates were generated for each treatment group (non-stretched or stretched).

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Project description:Time-course of gene profiles indicate that gene expression changing in cyclic stretch are related to cell adhesion and MAPK signaling. Gene expression changing by uniaxiali stretch was studied in a time course assay within 1.5 hour. Six time points (0, 10, 20, 30, 60, 90 min) were triplicated

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Rearrangement of actin stress fiber in HUVECs under cyclic stretch

Project description:Time-course of gene profiles indicate that gene expression changing in cyclic stretch are related to cell adhesion and MAPK signaling.

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Project description:With gene expression profiling it was aimed to identify the differentially expressed genes associated with the regulation of the cytoskeleton to investigate the stretch-induced cell alignment mechanism. A whole genome microarray based analysis of the stretch-induced gene expression changes was done. Gene expression was measured at the beginning of the alignment process showing first reoriented cells after 5 h stretching and at the end after 24 h, where nearly all cells are aligned. Cyclic mechanical stretching of cells results in cellular alignment perpendicular to the stretch direction regulating cellular response. This stress response is assumed to be an adaptation mechanism to reduce extensive stretching but also acts as architectural restructuring changing performance and biomechanics of the tissue. Gene expression profiling of control vs. stretched primary human dermal fibroblasts after 5 h and 24 h demonstrated the regulation of differentially expressed genes associated with metabolism, differentiation and morphology.

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Cyclic mechanical stretch-induced gene expression

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Expression data from Bronchial Epithelial Cells exposed to Cyclic Stretch with and without LPS or TNFa

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