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The Trihelix Transcription Factor GT2-like 1 (GTL1) promotes salicylic acid metabolism and regulates basal and bacterial effector-triggered Immunity

ABSTRACT: The Trihelix Transcription Factor GT2-like 1 (GTL1) promotes salicylic acid metabolism and regulates basal and bacterial effector-triggered Immunity

PROVIDER: PRJNA486344 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7703201_1.fastq.gz	Fastqsanger.gz
	SRR7703201_2.fastq.gz	Fastqsanger.gz
	SRR7703202_1.fastq.gz	Fastqsanger.gz
	SRR7703202_2.fastq.gz	Fastqsanger.gz
	SRR7703193_1.fastq.gz	Fastqsanger.gz

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The Trihelix Transcription Factor GT2-like 1 (GTL1) promotes salicylic acid metabolism and regulates basal and bacterial effector-triggered Immunity

Project description:We report the transcriptome composition of gtl1-2 compared to Columbia wild-type plant without treatment and after flg22-application

2018-10-05 | GSE118651 | GEO

The Trihelix transcription factor GT2-like 1 (GTL1) promotes salicylic acid metabolism, and regulates bacterial-triggered immunity.

Project description:The Trihelix Transcription factor GT2-like 1 (GTL1) was previously shown to be a key regulator of ploidy-dependent trichome growth and drought tolerance. Here, we report that GTL1 plays an important role in coordinating plant immunity. We show that gtl1 mutants are compromised in the regulation of basal immunity, microbial pattern-triggered immunity (PTI) and effector-triggered RIN4-mediated immunity. Transcriptome analysis revealed that GTL1 positively regulates defense genes and inhibits factors that mediate growth and development. By performing hormonal measurements and chromatin-immunoprecipitation studies, we found GTL1 to coordinate genes involved in salicylic acid metabolism, transport and response. Interaction studies and comparative transcriptomics to known data sets revealed that GTL1 is part of the MPK4 pathway and regulates oppositely the expression of differentially expressed genes in mpk4 plants. We introduced the gtl1 mutation in the mpk4 mutant and thereby partially suppressed its dwarfism and the high resistance against a bacterial invader. Our data show that GTL1 is part of the MPK4 pathway and acts as a positive regulator of bacterial-triggered immunity and SA homeostasis.

| S-EPMC6198943 | biostudies-literature

An immune-inducible trihelix transcription factor suppresses bacterial resistance in Arabidopsis thaliana

Project description:The plant innate immunity consists of the two interconnected mechanisms, pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). Although much is known about how plants trigger immune responses upon pathogen recognition, the genetic program by which plants avoid an overshoot in pathogen-triggered immune responses remains largely unknown. Here, we discovered a trihelix transcription factor, GT-3a, as an immune-inducible negative regulator of bacterial resistance in Arabidopsis thaliana. Analysis of public RNA-seq data revealed that GT-3a is specifically induced by ETI activation not by PTI activation. Overexpression of GT-3a suppressed resistance against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pto) and Pto-elicited expression of salicylic acid (SA)-responsive genes. Our results suggest that transcriptional induction of GT-3a circumvents the overshooting of SA-mediated defense responses during ETI.

2024-02-18 | GSE248054 | GEO

Root hair specific transcriptome analysis of gain-of-function and loss-of-function of GTL1 and DF1

Project description:How plants determine the final size of growing cells is an important, yet unanswered question. Root hairs provide an excellent model system to study this question since their final cell size is remarkably constant under given environmental conditions. In this study we demonstrate that a trihelix transcription factor GT-2-LIKE1 (GTL1) and its homolog DF1 repress root hair growth in Arabidopsis. Our transcriptional data, combined with genome-wide chromatin binding data, show that GTL1 and DF1 directly bind the RSL4 promoter and regulate its expression to repress root hair growth. Our data further show that GTL1 and RSL4 regulate each other as well as a set of common downstream genes, many of which have previously been implicated in root hair growth. This study therefore uncovers a core regulatory module that fine-tunes the extent of root hair growth by orchestrated actions of opposing transcription factors.

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Genome-wide GTL1 binding sites in Arabidopsis thaliana

Project description:To understand how GTL1 regulates cell growth, we first identified its potential direct targets by the chromatin immunoprecipitation followed by the hybridization on an Affymetrix Arabidopsis Tiling 1.0R array (ChIP-chip). To enrich the genomic region bound by GTL1 in vivo, we harvested whole aerial parts of 12-day-old gtl1-1 plants complemented with the pGTL:GTL1:GFP constructs and immunoprecipitated the chromatin fragments associated with GTL1-GFP proteins using antibodies against GFP. After applying a cut-off P-values of 0.001of MAT (Model-based analysis of tiling array), we identified a total number of 3,900 putative immediate target genes that showed consistent binding by GTL1.

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Genome-wide GTL1 binding sites in Arabidopsis thaliana

2013-08-01 | E-GEOD-40519 | biostudies-arrayexpress

Transcriptional repression of GTL1 by water-deficit stress promotes anthocyanin and polyamine biosynthesis to enhance drought tolerance

Project description:GT2-LIKE 1 (GTL1) is a negative regulator of stomatal development and its repressed expression under water deficit can result in enhanced drought tolerance. As a transcription factor, GTL1 has been implicated in diverse developmental roles. We hypothesized that GTL1 represses multiple drought tolerance pathways, leading to the drought tolerance phenotype of the gtl1-4 knockout mutant. RNA-Seq data indicates that GTL1 regulates genes involved in ribosome biogenesis in emerging leaves and secondary metabolite synthesis in expanding leaves.

2022-07-25 | GSE209584 | GEO

Genome-wide biding assay of GTL1 and DF1

Project description:We performed a chromatin immunoprecipitation-based microarray experiment (ChIP chip) in order to identify GTL1 and DF1 direct target genes

2018-02-15 | GSE104010 | GEO

mRNA profiling of Trihelix transcription factor ASIL mutants in seedlings

Project description:The current study aimed at exploring the roles of ASIL1 and ASIL2 trihelix transcription factors in repressing the maturation program during seedling development

2021-08-26 | GSE163006 | GEO

Comparison of gene expression in Arabidopsis 5th and 6th rosette leaves between gtl1-1 mutant and wild type

Project description:Total RNA from trichomes of fifth and sixth rosette leaves of three-week-old wild-type and gtl1-1 mutants (Figure 3B) were extracted. We found a total number of 1,759 genes, corresponding to 1,694 probes on the ATH1 chip, that show differential expression of at least 1.3-fold. Out of these 1,694 genes, 47.2% are positively regulated and 52.8% are negatively regulated by GTL1. Compared gene expressions in fifth and sixth rosette leaves of three-week-old wild-type and gtl1-1 mutants

2013-08-01 | E-GEOD-40518 | biostudies-arrayexpress

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