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Transcriptome analysis of Sinorhizobium meliloti strain ectopically overexpressing trpL

ABSTRACT: Transcriptome analysis of Sinorhizobium meliloti strain ectopically overexpressing trpL

PROVIDER: PRJNA486461 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7704920.fastq.gz	Fastqsanger.gz
	SRR7704921.fastq.gz	Fastqsanger.gz
	SRR7704922.fastq.gz	Fastqsanger.gz
	SRR7704923.fastq.gz	Fastqsanger.gz
	SRR7704924.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of Sinorhizobium meliloti strain ectopically overexpressing trpL

Project description:We show that the RNA leves of many genes in the S. meliloti overexpressing strain (OE) 2011 (pRK-RcsR1) are more similar to the levels of in wild type (WT) strain 2011 than in the empty vector control (EVC) strain 2011(pRK4352). Since the plasmid-containing strains were grown in the presence of 10 µg/ml tetracycline (Tc), while the WT was grown without Tc, the results suggest that the intrecellular Tc concentration in the OE strain are lower than in the EVC. This is explained by an increased efflux pump activity in the OE strain.

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Project description:We characterized transcriptomes for strains overexpressing each of the Sinorhizobium meliloti ECF sigma factors the via a plasmid-borne, melibiose-inducible promoter plasmid (PmelA; pCAP11: Pinedo et al. 2008 J Bacteriol 190:2947-2956) compared to control strains carrying the empty vector.

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Identification of RpoE2 target genes in Sinorhizobium meliloti

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