Project description:Vibrio parahaemolyticus is a Gram-negative bacterium commonly found in marine and estuarine environments. Acute hepatopancreatic necrosis disease (AHPND) caused by this bacterium is an ongoing problem among shrimp farming industries. V. parahaemolyticus proteins PirA and PirB have been determined to be major virulence factors that induce AHPND. In this study, Pacific white shrimp (Litopenaeus vannamei) were challenged with recombinant PirA and PirB by a reverse gavage method and then at 30 m, 1, 2, 4, and 6 h time points, the hepatopancreas of five individual shrimp were removed and placed into RNA later. We conducted RNA sequencing of the hepatopancreas samples from a no PirA/B control (n = 5) and PirA/B-treated shrimp at the different time intervals (n=5). We evaluated the different gene expression patterns between the time groups to the control with a focus on identifying differences in innate immune function.
Project description:The Pacific white shrimp (Litopenaeus vannamei), one of the most widely cultured shrimp species in the world, usually suffered from chilling stress in China. In order to reveal inner proteomics mechanism of chilling tolerance, we conducted a proteomic analysis on two contrasting shrimp cultivars, namely, chilling-tolerant GH2 and chilling-sensitive GH1 under normal temperature 28°C, chilling stress 16°C, and 16°C recovered to 28°C, respectively.
2018-05-25 | PXD009889 |
Project description:Intestinal microbiota of shrimp
