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Transcriptomic comparison of Listeria monocytogenes EGDe and its ΔlftR mutant

ABSTRACT: Transcriptomic comparison of Listeria monocytogenes EGDe and its ΔlftR mutant

PROVIDER: PRJNA486778 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7721750_1.fastq.gz	Fastqsanger.gz
	SRR7721750_2.fastq.gz	Fastqsanger.gz
	SRR7721745_1.fastq.gz	Fastqsanger.gz
	SRR7721745_2.fastq.gz	Fastqsanger.gz
	SRR7721746_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

In vivo transcriptome of Listeria monocytogenes EGDe

Project description:Time course study of the mouse infection by comparing the genomic transcriptional patterns of Listeria monocytogenes EGDe grown under laboratory conditions (exponential growth phase) with that of in vivo-grown bacteria (in mouse spleens) over three days of infection. Time course study of the mouse infection by comparing the genomic transcriptional patterns of Listeria monocytogenes EGDe grown under laboratory conditions (exponential growth phase) with that of in vivo-grown bacteria (in mouse spleens) over three days of infection.

2010-06-25 | E-GEOD-13057 | biostudies-arrayexpress

In vivo transcriptome of Listeria monocytogenes EGDe

2009-05-29 | GSE13057 | GEO

Transcriptomic comparison of Listeria monocytogenes EGDe and its Δlmo0599 mutant.

Project description:Transcriptomic comparison of Listeria monocytogenes EGDe and its Δlmo0599 mutant.

| PRJNA533134 | ENA

Transcriptomic comparison of Listeria monocytogenes EGDe and its Δlmo1408 mutant.

Project description:Transcriptomic comparison of Listeria monocytogenes EGDe and its Δlmo1408 mutant.

| PRJNA533123 | ENA

Transcriptomic comparison of Listeria monocytogenes EGDe and its Δlmo0422 mutant.

Project description:Transcriptomic comparison of Listeria monocytogenes EGDe and its Δlmo0422 mutant.

| PRJNA533128 | ENA

Transcriptomic profiling of L-rhamnose utilization in Lmo0753 deletion mutant of Listeria monocytogenes EGDe from human foodborne outbreak associated lineage LII

Project description:Among the three major genetic lineages of L. monocytogenes (i.e. LI, LII, and LIII), LI and LII are predominantly associated with foodborne listeriosis outbreaks, whereas LIII is rarely implicated in human infections. In a previous study, we identified a Crp/Fnr family transcription factor lmo0753 that was highly specific to outbreak-associated LI and LII but absent from LIII. Lmo0753 shares two conserved functional domains including a DNA-binding domain with the well-characterized master virulence regulator PrfA in L. monocytogenes. In this study, we constructed a lmo0753 deletion and complementation mutants of the fully sequenced L. monocytogenes LII strain EGDe. We found that deletion of lmo0753 led to the loss of L-rhamnose utilization in EGDe. Transcriptomic comparison of the EGDe lmo0753 deletion mutant and the wild type incubated in phenol-red medium containing L-rhamnose as the sole carbon source revealed 126 (4.5%) and 546 (19.5%) out of 2,798 genes in the EGDe genome that were up- and down-regulated for more than 2-fold, respectively. Genes involved in biotin biosynthesis, general stress response and rhamnose metabolism were shown to be differentially regulated by Lmo0753. Findings from this study may partially explain why LIII of L. monocytogenes is underrepresented in the environment and rarely associated with human listeriosis outbreaks due to the inability of rhamnose utilization. We report the transcriptomic profile of L. monocytogenes Δlmo0753 LII strain (EGDe) in broth media with L-rhamnose as the sole carbon source.

2013-07-17 | GSE47721 | GEO

Temperature dependent Transcriptome of L. monocytogenes EGDe in minimal medium

Project description:Comparison of transcriptome of L. monocytogenes EGDe at 24M-BM-0C and 37M-BM-0C L. monocytogenes EGDe cells used in this study were grown either at 24M-BM-0C or 37M-BM-0C to an OD600 = 0.82-0.87 and the transcriptome of these two conditions was compared Six biologically independent cultures of each at 24M-BM-0C and 37M-BM-0C grown L. monocytogenes EGDe were used for total RNA isolation (six RNA sets). Three array experiments were performed with Cy5-labelled cDNA derived from at 24M-BM-0C grown Listeria and Cy3-labelled cDNA derived from at 37M-BM-0C grown cells. For the three other RNA sets a dye swap was performed, that means the three other array experiments were performed with Cy3-labelled cDNA derived form at 24M-BM-0C grown Listeria and Cy5-labelled cDNA from at 37M-BM-0C grown cells. As all oligonuceotides are spotted twice on an array, technical duplicates were performed for each experiment. The overall design results in 12 data points for the expression of each gene.

2014-05-23 | E-GEOD-51193 | biostudies-arrayexpress

Temperature dependent Transcriptome of L. monocytogenes EGDe in minimal medium

Project description:Comparison of transcriptome of L. monocytogenes EGDe at 24°C and 37°C L. monocytogenes EGDe cells used in this study were grown either at 24°C or 37°C to an OD600 = 0.82-0.87 and the transcriptome of these two conditions was compared

2014-05-23 | GSE51193 | GEO

Transcriptomic profiling of L-rhamnose utilization in Lmo0753 deletion mutant of Listeria monocytogenes EGDe from human foodborne outbreak associated lineage LII

Project description:Among the three major genetic lineages of L. monocytogenes (i.e. LI, LII, and LIII), LI and LII are predominantly associated with foodborne listeriosis outbreaks, whereas LIII is rarely implicated in human infections. In a previous study, we identified a Crp/Fnr family transcription factor lmo0753 that was highly specific to outbreak-associated LI and LII but absent from LIII. Lmo0753 shares two conserved functional domains including a DNA-binding domain with the well-characterized master virulence regulator PrfA in L. monocytogenes. In this study, we constructed a lmo0753 deletion and complementation mutants of the fully sequenced L. monocytogenes LII strain EGDe. We found that deletion of lmo0753 led to the loss of L-rhamnose utilization in EGDe. Transcriptomic comparison of the EGDe lmo0753 deletion mutant and the wild type incubated in phenol-red medium containing L-rhamnose as the sole carbon source revealed 126 (4.5%) and 546 (19.5%) out of 2,798 genes in the EGDe genome that were up- and down-regulated for more than 2-fold, respectively. Genes involved in biotin biosynthesis, general stress response and rhamnose metabolism were shown to be differentially regulated by Lmo0753. Findings from this study may partially explain why LIII of L. monocytogenes is underrepresented in the environment and rarely associated with human listeriosis outbreaks due to the inability of rhamnose utilization. We report the transcriptomic profile of L. monocytogenes M-NM-^Tlmo0753 LII strain (EGDe) in broth media with L-rhamnose as the sole carbon source. Examination of deletion of Lmo0753 on L-rhamnose utilization in L. monocytogenes. Two biological replicates per WT and M-NM-^Tlmo0753.

2013-07-17 | E-GEOD-47721 | biostudies-arrayexpress

Analysis of σL dependent traits based on phenotypic and transcriptomic evaluation of the Listeria monocytogenes EGDe strain

Project description:The phenotypic and gene expression traits conferred by the alternative sigma factor protein σL in the food-borne pathogen L. monocytogenes were investigated. σL was shown to be important for the efficient growth of this pathogen exposed to food preservative measures such as low storage temperatures, elevated osmolarity and acidity. Based on high throughput phenotypic analysis, σL function was also found to be protective in L. monocytogenes EGDe cells exposed to several antimicrobial compounds including some of the antibiotics currently applied in listeriosis treatment. The expression of flagella genes and motility were significantly compromized upon loss of σL function. A comparative transcriptome analysis of exponential growth EGDe wild type and sigL null cells unveiled 394 genes that are positively controlled through σL dependent transcriptional regulation mechanisms in this bacterium during growth at low (3°C) and optimized (37°C) temperature conditions. Genes identified indicate that σL is a pleitropic transcription regulator mediating positive expression control of genes involved in diverse cellular processes including protein synthesis, molecular transport, energy metabolism, respiration, transcription regulation, metabolite biosynthesis, and cell envelope composition modification. Overall our observations have revealed that the loss of σL function leads to extensive gene expression defects in L. monocytogenes EGDe cells, and these are consistent with compromized nutrient assimilation, energy metabolism, protein synthesis, and metabolite biosynthesis processes as well as altered cell envelope composition and motility. Numerous gene expression changes imposed by σL loss in EGDe are thus also consistent with pleiotropic phenotypic defects detected in the L. monocytogenes EGDe ∆sigL strain.

2012-01-01 | GSE32434 | GEO

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