Project description:Investigation of whole genome gene expression level changes in Candida tenuis NRRL Y-1498 grown aerobically in xylose, compared to the same strain grown aerobically in glucose.
Project description:Investigation of whole genome gene expression level changes in Lodderomyces elongisporus NRRL YB-4239 grown aerobically in xylose, compared to the same strain grown aerobically in glucose.
Project description:Investigation of whole genome gene expression level changes in Spathaspora passalidarum NRRL Y-27907 grown aerobically in xylose, compared to the same strain grown aerobically in glucose.
Project description:Myxobacteria are social bacteria distinguished among prokaryotes by a multicellular stage in their life cycle known as fruiting bodies formations, which is a response to nutrient deprivation. Additionally, fruiting body formation in Stigmatella aurantiaca is stimulated by light. Genome sequence of Myxococcus macrosporus, revealed the presence of a red-light photoreceptor, bacteriophytochrome (BphP), found in photosynthetic and non-photosynthetic bacteria. BphPs belong to a large phytochrome family of photoreceptors originally discovered in plants, where they are directly linked to the circadian clock genes. BphPs have been implicated in carotenoid synthesis, motility, and photodynamic stress in non-photosynthetic bacteria. The role of the BphPs in M. macrosporus remains unknown although it has been linked to fruiting body formation in related S. aurantiaca. We have observed that M. macrosporus displays differential photomorphogenic behaviors under constant light, and 12:12 hour light/dark (LD) cycle as compared to constant dark (DD) conditions. We hypothesize that BphP plays a role in these behaviors. In this study we demonstrate light and nutrient dependent pattern of fruiting bodies development in Myxococcus macrosporus HW-1. Additionally, we characterized light induced differential gene expression with the special focus on the circadian clock, and carotenoid biosynthesis genes in M. macrosporus and M. xanthus.