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The genome-wide enhancer profiling of breast cancer in the MMTV-PyVT mice

ABSTRACT: The genome-wide enhancer profiling of breast cancer in the MMTV-PyVT mice

PROVIDER: PRJNA486844 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7723990_1.fastq.gz	Fastqsanger.gz
	SRR7723990_2.fastq.gz	Fastqsanger.gz
	SRR7724000_1.fastq.gz	Fastqsanger.gz
	SRR7724000_2.fastq.gz	Fastqsanger.gz
	SRR7724160_1.fastq.gz	Fastqsanger.gz

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The genome-wide enhancer profiling of breast cancer in the MMTV-PyVT mice [ChIP-seq]

Project description:Activation of transcription enhancers, especially super enhancers, is one of the important epigenetic features of tumorigenesis. However, only very few studies reported how the enhancer landscape evolves during tumorigensis. Here we utilized a proteomics approach and found that H3K27ac and H4K8ac are elevated in the mammary tumor of MMTV-PyVT mouse model, which was then confirmed by IHC results in human BRCA chips. ChIP-seq studies revealed that H4K8ac is highly co-localized with H3K27ac on chromatin, especially on the distal enhancers . We further identified a subgroup of super enhancers which are marked by H3K4me3 peaks in the intergenic regions. These H3K4me3-enriched super enhancers are covered with higher level of H3K27ac and H4K8ac in tumor than normal tissue , and accompanies higher transcription level of the adjacent genes. Combined with global gene expression data, we identified 148 tumor H3K4me3-enriched super enhancer adjacent genes with higher expression in the tumor tissues , which may be critical for breast cancer. We further utilized one inhibitor of p300, C646, to inhibit H3K27ac level, which successfully repressed tumor formation process probably through inhibiting Vegfa and other oncogenes. Taken together, our work identifies novel regulators and provides important resource to the genome-wide enhancer studies in breast cancer, and raises the possibility of cancer treatment through modulating enhancer activity.

2019-08-18 | GSE118810 | GEO

The genome-wide enhancer profiling of breast cancer in the MMTV-PyVT mice [RNA-seq]

2019-08-18 | GSE118802 | GEO

The genome-wide enhancer profiling of breast cancer in the MMTV-PyVT mice [ChIP-seq]

Project description:The genome-wide enhancer profiling of breast cancer in the MMTV-PyVT mice [ChIP-seq]

| PRJNA486843 | ENA

The genome-wide enhancer profiling of breast cancer in the MMTV-PyVT mice [RNA-seq]

Project description:The genome-wide enhancer profiling of breast cancer in the MMTV-PyVT mice [RNA-seq]

| PRJNA486836 | ENA

RNA analysis of Nr4a1 and Nr4a1-knockout mammary tumor cells from MMTV-PyVT transgenic mice

Project description:MMTV-PyVT Mammary tumors were harvested from PyVT-wild-type (WT), PyVT-Nr4a1 knockout (KO) littermates on a congenic FVB/NJ background,minced and incubated with collagenase type I digestion solution .The digested Mammary tumor cells were collected and maintained in DMEM medium containing 10% FBS for 24 hour.

2020-10-01 | GSE131895 | GEO

miRNA expression during early, mid and late breast cancer disease progression in mammary glands from MMTV-PyMT transgenic mice

Project description:MicroRNAs (miRNAs) are small noncoding RNAs that typically inhibit the translation and stability of messenger RNAs (mRNAs), controlling genes involved in a variety of cellular processes. miRNA dysregulation is recognized to play an essential role in the development and progression of cancer. MMTV-PyMT mice (Jax Strain: FVB/N-Tg(MMTV-PyVT)634Mul/J) are a well-characterized transgenic mouse model of breast cancer. Upon activation of the MMTV-PyVT transgene (mouse mammary tumor virus (MMTV) long terminal repeat upstream of a cDNA sequence encoding the Polyoma Virus middle T antigen (PyVT)) female carriers develop palpable mammary tumors as early as 5 weeks of age. We performed miRNA microarrays on samples from the MMTV-PyMT transgenic mouse model to investigate the differential expression of miRNAs during development of malignant disease in this model.

2017-08-23 | GSE68630 | GEO

The genome-wide enhancer profiling of breast cancer in the MMTV-PyVT mice

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-08-18 | GSE118812 | GEO

Differential chromatin accessibility and transcriptional dynamics define breast cancer subtypes and their lineages

Project description:To explore pre-cancer states during early malignancy, we evaluated tumor and normal cells in the MMTV-PyVT mouse model of luminal breast cancer using snRNAseq and snATACseq.

2023-08-10 | GSE240577 | GEO

Abi1 gene dose study in PyVT breast tumors

Project description:Abi1 gene dose study in PyVT breast tumors

| PRJNA683072 | ENA

Huntington disease protein accelerates tumour development and metastasis

Project description:Huntington disease is a severe neurological disorder caused by an abnormal polyglutamine expansion in the N-terminal of the huntingtin protein. Here we show that breast tumours appear earlier when mutant huntingtin is expressed in an activated polyomavirus middle T antigen (PyVT) mouse breast cancer model as compared to the control mice expressing wild-type huntingtin. Tumours bearing mutant huntingtin have a modified gene expression pattern revealing increases in the IGF-1/Akt signalling, epithelial-mesenchymal transition and metastatic properties. Indeed, polyQ-huntingtin expressing tumours show hyper-activation of Akt pathway. Also, when mutant huntingtin is expressed, cancer cells in culture change morphology and the levels of cell adhesion and mesenchymal markers are affected in primary tumour or corresponding derived cells. As a consequence, PyVT induced lung metastasis is higher in Huntington disease mice than in the control mice. Finally, analysis of cases of Huntington disease patients developing breast cancer may suggest an increased aggressiveness of breast cancer when compared to the control population. 8 Total samples were analyzed: 4 x MMTV-PyVT/HdhQ7/Q7 breast tumours; 4 x MMTV-PyVT/HdhQ111/Q111 breast tumours;

2014-04-14 | E-GEOD-28685 | biostudies-arrayexpress

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