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Expression data from mouse fetal and adult intestinal organoid cultures and intestinal tissues

ABSTRACT: Expression data from mouse fetal and adult intestinal organoid cultures and intestinal tissues

PROVIDER: PRJNA487610 | ENA |

REPOSITORIES: ENA

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Expression data from mouse fetal and adult intestinal organoid cultures and intestinal tissues

Project description:Here we profiled fetal intestinal epithlelium derived organoids at day 3 (group1) and 30 (group2) of culture, adult orgnanoids at day 3 (group3) and 30 (group4) of culture and whole intestinal tissues at P0(group5) and adult (group6).

2018-12-01 | GSE118982 | GEO

Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration

Project description:Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration

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Use of Human Adult Stem Cell-Derived Organoid Cultures to Model Enterohepatic Circulation

Project description:Organ to organ interactions have not yet been modeled with human organoid cultures. This work demonstrates the coupling of intestinal and liver organoids recapitulates aspects of enterohepatic bile signaling. The integration of organoids can be used to probe intra-organ interactions. Single cell RNA-Seq studies were performed in human liver organoids to examine the heterogeneity of the cultures.

2021-07-28 | GSE166589 | GEO

Development of Plasmodium falciparum liver stages in human fetal hepatocyte organoid cultures

Project description:Development of Plasmodium falciparum liver stages in human fetal hepatocyte organoid cultures

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Use of Human Adult Stem Cell-Derived Organoid Cultures to Model Enterohepatic Circulation [Ileal organoids]

Project description:Organ to organ interactions have not yet been modeled with human organoid cultures. This work demonstrates the coupling of intestinal and liver organoids recapitulates aspects of enterohepatic bile signaling. The integration of organoids can be used to probe intra-organ interactions. Total RNA-Seq studies were performed in human ileal organoids to examine expression of bile acid signaling molecules.

2021-08-21 | GSE168005 | GEO

Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration [scRNA-seq]

Project description:Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration [scRNA-seq]

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Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration [ChIP-seq]

Project description:Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration [ChIP-seq]

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Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration [scRNA-seq]

Project description:The capacity of 3D organoids to mimic physiological tissue organization and functionality has provided an invaluable tool to model development and disease in vitro. However, conventional organoid cultures primarily represent the homeostasis of self-organizing including VPA, EPZ6438, LDN193189, and R-Spondin 1 conditioned medium, which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury; therefore, these organoids were designated hyperplastic intestinal organoids (Hyperorganoids). Single-cell RNA sequencing identified different regenerative stem cell populations in our Hyper-organoids that shared molecular features with in vivo injury-responsive Lgr5+ stem cells or Clu+ revival stem cells. Further analysis revealed that VPA and EPZ6438 were indispensable for epigenome reprogramming and regeneration in Hyper-organoids, which functioned through epigenetically regulating YAP signaling. Furthermore, VPA and EPZ6438 synergistically promoted regenerative response in gut upon damage in vivo. In summary, our results demonstrated a new in vitro organoid model to study epithelial regeneration, highlighting the importance of epigenetic reprogramming that pioneers tissue repair.

2021-01-20 | GSE165092 | GEO

Establishment of intestinal organoid cultures modeling injuryassociated epithelial regeneration [ChIP-seq]

2021-01-16 | GSE164940 | GEO

Single cell RNA sequencing of intestinal fibroblast-crypt co-cultures in organoid growth media with/without Ptger4/EP4 inhibitor (Drop-seq)

Project description:Mouse small intestinal crypts were co-cultured with primary intestinal fibroblasts in Organoid Growth Media (OGM) with/without inhibitor for the prostaglandin E2 receptor Ptger4 (EP4), labeled respectively as “Ptger4-OFF” and “Ptger4-ON” conditions. Crypts co-cultured with fibroblasts in Ptger4-ON conditions (OGM containing DMSO vehicle control) acquired a spheroid morphology. Crypts co-cultured with fibroblasts in Ptger4-OFF conditions (OGM supplemented with Ptger4 inhibitor) developed into budding organoids. Fibroblasts were isolated from the small intestine of wild type mice. For the co-cultures 2 x 10e4 fibroblasts were seeded in 48-well plates overnight. Freshly isolated crypts (n = 500) were suspended in 1:1 Matrigel (Corning, 356231) and IntestiCult Organoid Growth Medium (Stem Cell Technologies, 06005) and added as an overlay on the fibroblasts. Crypts/fibroblasts were co-cultured with IntestiCult Organoid Growth Medium for 4 days. The ONO-AE3-208 Ptger4 (EP4) inhibitor (Cayman, 14522) dissolved in DMSO was added to the co-cultureson days 0 and 2 at a final concentration of 10 uM. DMSO was used as a vehicle control for the untreated co-cultures. On day 4, one pool of six Ptger4-ON co-cultures and one pool of six Ptger4-OFF co-cultures were subjected to the Drop-seq protocol.

2019-12-21 | GSE142420 | GEO

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