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Transcriptome analysis of influenza infected GFP+ AEC compared to bystander GFP- AEC

ABSTRACT: Transcriptome analysis of influenza infected GFP+ AEC compared to bystander GFP- AEC

PROVIDER: PRJNA488226 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7761180.fastq.gz	Fastqsanger.gz
	SRR7761181.fastq.gz	Fastqsanger.gz
	SRR7761182.fastq.gz	Fastqsanger.gz
	SRR7761183.fastq.gz	Fastqsanger.gz
	SRR7761174.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of influenza infected GFP+ AEC compared to bystander GFP- AEC

Project description:A GFP-expressing recombinant A/Puerto Rico/8/1934 influenza virus was used to infect C57BL/6 wild type mice and on day 3 post infection, lung alveolar epithelial cells (AEC) were isolated and sorted based on GFP expression. GFP+ AEC represent the infected AEC and GFP- AEC represent the bystander AEC. AEC were also sorted from uninfected mice to serve as controls.

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Project description:Our previous data obtained by using immunohistochemistry showed, that Fgf10+/- (50% Fgf10 expression compared to WT) in hyperoxic condition at postnatal day 3 (P3) compared to WT has less vessel count in the lung and less muscularization of small capillaries in the lung. Furthermore, Fgf10+/- showed a drastic increase in mortality upon hyperoxic lung injury. Main question to be answer by this experiment is as followed: Does AEC II from Fgf10+/- mice in normoxia show different expression profiles at P3 compared to AEC II from WT? To adress this question we harvest lungs at P3 from WT and Fgf10+/- . For this the mice were sacrificed by Ketamin/ Dormitor ip, lungs were perfused transcardiac with PBS and directly frozen in liquid nitrogen.

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Enterobacter chuandaensis strain:AEC

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