Project description:The redox-sensing two-component signal transduction system, RegSR, in Rhodopseudomonas palustris has been shown to regulate an uptake hydrogenase in response to varying cellular redox states; however, its role is still largely undefined. Here, we used RNA sequencing to compare gene expression patterns in wild type R. palustris strain CGA010 to a ΔregSR derivative, CGA2023, under varying metabolic conditions. Growth conditions were chosen to utilize the different metabolic capabilites of R. palustris and, thus, present a variety of different redox challenges to the cell.
Project description:In this paper, we present the first comparative transcriptome profiles with ARR treated and control of R. palustris. Moreover, putative two ARR biotransformation mechanisms in R. palustris were first given. All of these provided a valuable genomic resource for further studying molecular mechanism of biotransformation and genetic modification of R. palustris.