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ABSTRACT: Coupling Enhancement of Chromium(VI) Bioreduction in Groundwater by solid phosphorus minerals

PROVIDER: PRJNA488509 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7774415_1.fastq.gz	Fastqsanger.gz
	SRR7774415_2.fastq.gz	Fastqsanger.gz
	SRR7774416_1.fastq.gz	Fastqsanger.gz
	SRR7774416_2.fastq.gz	Fastqsanger.gz

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Project description:Coupling Enhancement of Chromium(VI) Bioreduction in Groundwater by solid phosphorus minerals

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Global transcriptional analysis of Cr(VI)-adapted cells of Klebsiella sp. AqSCr

Project description:Hexavalent chromium (Cr(VI)) is a highly toxic contaminant, some bacteria are able to transform it to less toxic and less soluble trivalent chromium (Cr(III)). Klebsiella sp. strain AqSCr, isolated from Cr(VI)-polluted groundwater, reduces Cr(VI) both aerobically and anaerobically, and resists up 35 mM of Cr(VI); Subculturing of AqSCr in the presence of Cr(VI) conduces to adaptation. In this study, we performed RNA-Seq of Cr(VI) adapted stage, finding 255 genes upregulated and 240 downregulated with respect to controls without Cr(VI). Genes differentially expressed are mostly associated with oxidative stress response, DNA repair and replication, sulfur starvation response, envelope-osmotic stress response, fatty acid metabolism, ribosomal subunits and energy metabolism. Among them, genes not previously associated with chromium resistance as cybB, encoding a putative superoxide oxidase, gltA2, encoding an alternative citrate synthase, and des, encoding a fatty acid desaturase were upregulated. The alternative sigma factors fecl, rpoE and rpoS were upredgulated in Cr(VI) adapted cells, then they participate in orchestate the Cr(VI)-resistance mechanisms in AqSCr strain

2021-05-20 | GSE160968 | GEO

Project description:Feasibility and Mechanism of Microbial-Phosphorus Minerals-Alginate Immobilized Particles in bioreduction of Cr(VI) and synchronous removal of Cr(III)

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Project description:Enhancement effect of minerals on microbial simultaneous nitrate and Cr(VI) reduction

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Chromium stress response in rice roots: effects on transcriptome proﬁles and signalling pathways responsive

Project description:Chromium (Cr) is an important environmental contaminant and also a genotoxic metal ion at high concentration, but its molecular mechanisms and signalling pathways of action in plants is still poorly unraveled. To help characterize the mechanism of molecular and signalling of rice, we present the large-scale, transcriptomic analysis of rice root responses to Cr(VI). We examined the possible involvement of reactive of reactive oxygen species (ROS) and calcium in Cr(VI) signaling transduction pathways, as well as the effect of Cr (VI) on CDPK and MAPK activity. Specially, we used the microarray assay to assess different stage Cr (VI) induced alteration in rice gene expression. This gene discovery effort will help expand our understanding of cellular responses to Cr (VI) treatment, and will identify candidate genes for enhancement of Cr resistance in crop.

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