Project description:To investigate the temperature response in Thermosynechococcus elongatus (BP-1) and look for coordinated control in the cell, transcriptomes of BP-1 were measured using RNAseq following exposure to low and high temperature stress. The amount of temperature increase (53 to 61 °C) and decrease (53 to 45 °C) was based on the allowable range of continuous growth. The cells were growth in three separate culture tubes under LED light in a temperature-controlled submersible chamber under batch growth conditions. The temperature shift was conducted once the cells reached their target concentration of 1.5E7 cells/ml. A control experiment was conducted, in which the temperature remained at 53 °C, ensuring that the cellular physiology and light attenuation were comparable and that the only difference between the hot and cold treatments relative to the control treatment was temperature.
Project description:The transcriptional response of the cyanobacteria T. elongatus to co-cultiavation with a heterotrophic partner M. ruber was examined under three varying light levels and three varying oxygen levels.
Project description:The transcriptional response of the cyanobacteria T. elongatus to co-cultiavation with a heterotrophic partner M. ruber was examined under three varying light levels and three varying oxygen levels.
Project description:A cyanobacterium, Thermosynechococcus elongatus BP1, was cultured with a heterotroph, Meiothermus ruber strain A, under a variety of conditions. A gene co-expression approach was used to infer a network of T. elongatus and M. ruber genes, with a focus on those genes from different species that were co-expressed, highlighting potential points of coordination and interaction.