Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Short RNAs associated to Cauliflower mosaic virus infections in Canola and Turnip

ABSTRACT: Short RNAs associated to Cauliflower mosaic virus infections in Canola and Turnip

PROVIDER: PRJNA489176 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7781230.fastq.gz	Fastqsanger.gz
	SRR7781227.fastq.gz	Fastqsanger.gz
	SRR7781228.fastq.gz	Fastqsanger.gz
	SRR7781229.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Short RNAs associated to Cauliflower mosaic virus infections in Canola and Turnip

Project description:Three plants of two species in Brassica genus, i.e. B. rapa cv. "Purple To White Globe" and B. napus cv. "SariGol", highly susceptible and tolerant hosts of Cauliflower mosaic virus (CaMV), respectively, were mechanically inoculated with CaMV strain NY8153.Phosphate buffer was used as control in mock-inoculated plants. RNAs, in three biological repeats, were extracted 20 days post-inoculation (dpi) collecting ten leaves from mock-inoculated and virus-infected plants using Trizol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. Five µg of total RNA were resolved in 15% SDS-PAGE gel. Total RNA pattern was visualized upon Ethidium Bromide staining and sRNAs in the size range of 20-30 nt were eluted from gel. sRNAs replicates were quantified and mixed in equal molarity. Libraries were obtained using NEXTflex Small RNA-Seq Kit v3 (Bio Scientific) according to manufacturer’s instructions. Libraries were eluted from a 6% non-denaturing PAGE. The quality of libraries was checked by BioPharma QC, and then, submitted for sequencing with a HiSeq 2500 Illumina platform.

2020-09-27 | GSE119398 | GEO

sRNAseq from Arabidopsis leaves non-infected and infected with Turnip mosaic virus (TuMV)

Project description:Sequencing of small RNAs from Arabidopsis leaves non-infected and infected with Turnip mosaic virus at 14 days post inoculation

2018-03-01 | GSE110940 | GEO

Turnip Yellow Mosaic Virus

Project description:Turnip Yellow Mosaic Virus Raw sequence reads

| PRJNA277612 | ENA

Turnip yellow mosaic virus

Project description:Turnip yellow mosaic virus Raw sequence reads

| PRJNA283239 | ENA

Transcriptome sequencing from Nicotiana benthamiana leaves non-infected and infected with Turnip mosaic virus (TuMV)

Project description:Transcriptome sequencing from Nicotiana benthamiana leaves non-infected and infected with Turnip mosaic virus at 6 days post inoculation.

2021-02-25 | GSE167415 | GEO

Transcription profiling of Arabidopsis Potyvirus turnip mosaic virus infection stages

Project description:A model system of Potyvirus turnip mosaic virus and Arabidopsis was used in this experiment. GFP-tagged virus supplied a visualized marker for us to localize the viral infection foci and its expansion on leaf under UV light. Initially, we dissect an individual infection focus and its adjacent region into four parts and define those four parts as zone 0, 1, 2, and 3, which represented different viral infection stages respectively. Corresponding fours parts were also dissected from control plant treated with turnip leaf sap only. This process was replicated three times totally.

2006-12-01 | E-MEXP-509 | biostudies-arrayexpress

Turnip mosaic virus

Project description:siRNAs 15-29 nucleotides long with 100 percent hit to Turnip mosaic virus clone GBR98

| PRJNA113381 | ENA

Arabidopsis LSM1 and DCP5 proteins aid evasion of translational repression during Cauliflower Mosaic Virus infection

Project description:Processing bodies are tightly connected with the RNA silencing machinery. Deletion of processing body components leads to succeptibility of Cauliflower Mosaic Virus to translational repression. Here, we investigated if this succeptibility is mediated through differential sRNA targeting of the viral genome.

2022-01-24 | GSE194186 | GEO

siRNAs 15-29 nucleotides long with 100 percent hit to Turnip mosaic virus clone GBR98

Project description:Mustard (Brassica juncea) was tested for Turnip mosaic virus infection. Small RNA of the plant was extracted and converted to DNA according to Ho, T., et al. (2006) Journal of Virological Methods 136:217-223, with primers modified to contain 454 adapter nucleotide sequences. The DNA then passed quality control through Bioanalyzer and Nanodrop before sequenced by 454 Life Sciences. Keywords: siRNA

2008-09-12 | GSE12053 | GEO

siRNAs 15-29 nucleotides long with 100 percent hit to Turnip mosaic virus clone GBR98

2008-09-11 | E-GEOD-12053 | biostudies-arrayexpress