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Generation of induced neural stem cells from urine derived cells by synthetic mRNA

ABSTRACT: Generation of induced neural stem cells from urine derived cells by synthetic mRNA

PROVIDER: PRJNA489883 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7807480_1.fastq.gz	Fastqsanger.gz
	SRR7807480_2.fastq.gz	Fastqsanger.gz
	SRR7807481_1.fastq.gz	Fastqsanger.gz
	SRR7807481_2.fastq.gz	Fastqsanger.gz
	SRR7807476_1.fastq.gz	Fastqsanger.gz

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Generation of induced neural stem cells from urine derived cells by synthetic mRNA

Project description:Analysis of induced neural stem cells from urine derived cells by synthetic mRNA vs. H9 embryonic stem cell derived neural stem cells and urine derived cells. Results provide insight into molecular similarities between induced neural stem cells and H9 embryonic stem cells derived neural stem cells.

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Efficient Generation of Integration-free and Engraftable induced Neural Stem Cells (iNSC) from Epithelial-like Cells in Human Urine

Project description:Human neural stem cells (NSCs) hold great promise in reparative therapy for neural diseases and injuries. However, the isolation of NSCs from either fetal or adult tissue and their application remain difficult due to ethical and immune-rejection concerns. One promising solution is to generate patient specific induced pluripotent stem cells (iPSCs) and subsequently differentiate them into NSCs. Alternatively, induced neurons (iN) can be generated directly from fibroblasts by retroviral delivery of neural specific transcription factors or miroRNAs. The later two approaches remain inefficient and with safety concerns. Here, we describe a process to generate, from epithelial-like cells present in human urine, integration free and engraftable NSCs (UiNSC) efficiently. Using an episomal system to deliver reprogramming factors and microRNAs into human urine cells and subsequently culture them in a chemically defined medium supplemented with a cocktail of small molecules, we generated UiNSCs capable of proliferation and without the transgenes upon prolonged expansion. These transgene free UiNSCs express typical neural stem cell markers and are able to differentiate into all neuronal subtypes and glial cells in vitro and more importantly could engraft and differentiate in vivo upon transplantation into the brain of new born rat. Thus, our work provides an efficient and highly practical platform to generate patient specific NSCs for regenerative medicine. This is a general expression microarray design (NimbleGen platform). It includes 6 samples.

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mRNA-Driven Generation of Transgene-Free Neural Stem Cells from Human Urine-Derived Cells.

Project description:Human neural stem cells (NSCs) hold enormous promise for neurological disorders, typically requiring their expandable and differentiable properties for regeneration of damaged neural tissues. Despite the therapeutic potential of induced NSCs (iNSCs), a major challenge for clinical feasibility is the presence of integrated transgenes in the host genome, contributing to the risk for undesired genotoxicity and tumorigenesis. Here, we describe the advanced transgene-free generation of iNSCs from human urine-derived cells (HUCs) by combining a cocktail of defined small molecules with self-replicable mRNA delivery. The established iNSCs were completely transgene-free in their cytosol and genome and further resembled human embryonic stem cell-derived NSCs in the morphology, biological characteristics, global gene expression, and potential to differentiate into functional neurons, astrocytes, and oligodendrocytes. Moreover, iNSC colonies were observed within eight days under optimized conditions, and no teratomas formed in vivo, implying the absence of pluripotent cells. This study proposes an approach to generate transplantable iNSCs that can be broadly applied for neurological disorders in a safe, efficient, and patient-specific manner.

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