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Shigella dysenteriae 225-75

ABSTRACT: Shigella dysenteriae 225-75 genome sequencing project

PROVIDER: PRJNA49065 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	AKNG01.dat.gz	Other
	AKNG01.fasta.gz	Fasta.gz
	AKNG01.master.dat	Other
	SRR364091.fastq.gz	Fastqsanger.gz
	SRR364091_1.fastq.gz	Fastqsanger.gz

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Project description:Small regulatory RNAs (sRNAs) of Shigella dysenteriae and other pathogens are vital for the regulation of virulence-associated genes and processes. Here, we characterize RyfA1, one member of a sibling pair of sRNAs produced by S. dysenteriae. Unlike its nearly identical sibling molecule RyfA2, predicted to be encoded almost exclusively by non-pathogenic species, the presence of a gene encoding RyfA1, or a RyfA1-like molecule, is strongly correlated with virulence in a variety of enteropathogens. In S. dysenteriae, the over production of RyfA1 negatively impacts the virulence-associated process of cell-to-cell spread and the expression of ompC, a gene encoding a major outer membrane protein important for the pathogenesis of Shigella. Interestingly, the production of RyfA1 is controlled by a second sRNA, here termed RyfB1; the first incidence of one regulatory small RNA controlling another in S. dysenteriae or any Shigella species.

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