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Shigella dysenteriae 225-75

ABSTRACT: Shigella dysenteriae 225-75 genome sequencing project

PROVIDER: PRJNA49065 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR364091.fastq.gz	Fastqsanger.gz
	SRR364091_1.fastq.gz	Fastqsanger.gz
	SRR364091_2.fastq.gz	Fastqsanger.gz

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Small RNA Sibling ryfA1 Influences Shigella dysenteriae Pathogenesis

Project description:Small regulatory RNAs (sRNAs) of Shigella dysenteriae and other pathogens are vital for the regulation of virulence-associated genes and processes. Here, we characterize RyfA1, one member of a sibling pair of sRNAs produced by S. dysenteriae. Unlike its nearly identical sibling molecule RyfA2, predicted to be encoded almost exclusively by non-pathogenic species, the presence of a gene encoding RyfA1, or a RyfA1-like molecule, is strongly correlated with virulence in a variety of enteropathogens. In S. dysenteriae, the over production of RyfA1 negatively impacts the virulence-associated process of cell-to-cell spread and the expression of ompC, a gene encoding a major outer membrane protein important for the pathogenesis of Shigella. Interestingly, the production of RyfA1 is controlled by a second sRNA, here termed RyfB1; the first incidence of one regulatory small RNA controlling another in S. dysenteriae or any Shigella species.

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