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NRDE2 negatively regulates nuclear exosome functions

ABSTRACT: NRDE2 negatively regulates nuclear exosome functions

PROVIDER: PRJNA491937 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7879110_1.fastq.gz	Fastqsanger.gz
	SRR7879110_2.fastq.gz	Fastqsanger.gz
	SRR7879120.fastq.gz	Fastqsanger.gz
	SRR7879120_1.fastq.gz	Fastqsanger.gz
	SRR7879120_2.fastq.gz	Fastqsanger.gz

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Similar Datasets

NRDE2 negatively regulates nuclear exosome functions

Project description:The exosome functions in the degradation of diverse RNA species, yet how it is negatively regulated remains largely unknown. Here, we show that NRDE2 forms a 1:1 complex with MTR4, a nuclear exosome cofactor critical for exosome recruitment, via a conserved MTR4-interacting domain (MID). Unexpectedly, NRDE2 mainly localizes in nuclear speckles, where it inhibits MTR4 recruitment and RNA degradation, and thereby ensures efficient mRNA nuclear export. Structural and biochemical data revealed that NRDE2 interacts with MTR4's key residues, locks MTR4 in a closed conformation, and inhibits MTR4 interaction with the exosome as well as proteins important for MTR4 recruitment, such as the cap-binding complex (CBC) and ZFC3H1. Functionally, MID deletion results in the loss of self-renewal of mouse embryonic stem cells. Together, our data pinpoint NRDE2 as a nuclear exosome negative regulator that ensures mRNA stability and nuclear export.

2019-03-14 | GSE120206 | GEO

NRDE2 negatively regulates nuclear exosome functions II

Project description:NRDE2 negatively regulates nuclear exosome functions II

| PRJNA491941 | ENA

NRDE2 negatively regulates nuclear exosome functions IV

Project description:NRDE2 negatively regulates nuclear exosome functions IV

| PRJNA491939 | ENA

NRDE2 negatively regulates nuclear exosome functions I

Project description:NRDE2 negatively regulates nuclear exosome functions I

| PRJNA491944 | ENA

NRDE2 negatively regulates nuclear exosome functions II

Project description:To examine whether NRDE2 can impact the competition between hMTR4 and ALYREF, we performed stranded RNA-seq to detect nuclear RNAs isolated from NRDE2 and control knockdown cells.

2019-03-14 | GSE120203 | GEO

NRDE2 negatively regulates nuclear exosome functions I

Project description:To examine whether NRDE2 can impact the competition between hMTR4 and ALYREF, we performed stranded RNA-seq to detect total RNAs isolated from NRDE2 and control knockdown cells.

2019-03-14 | GSE120202 | GEO

NRDE2 negatively regulates nuclear exosome functions IV

Project description:To examine whether NRDE2 can impact the competition between hMTR4 and ALYREF, we performed stranded RNA-seq to detect RNAs isolated from MTR4 IP in NRDE2 and control knockdown cells.

2019-03-14 | GSE120205 | GEO

Nuclear exosome

Project description:Nuclear exosome

| PRJNA304634 | ENA

NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.

| S-EPMC6499326 | biostudies-literature

3´-end sequencing of poly(A)+ RNA in wild-type Saccharomyces cerevisiae and nuclear exosome mutant strains

Project description:The nuclear exosome performs critical functions in non-coding RNA processing, and in diverse surveillance functions including the quality control of mRNP formation, and in the removal of pervasive transcripts. Most non-coding RNAs and pervasive nascent transcripts are targeted by the Nrd1p-Nab3p-Sen1p (NNS) complex to terminate Pol II transcription coupled to nuclear exosome degradation or 3´-end trimming. Prior to nuclear exosome activity, the Trf4p-Air2p-Mtr4p polyadenylation complex adds an oligo-A tail to exosome substrates. Inactivating exosome activity stabilizes and lengthens these A-tails. We utilized high-throughput 3´-end poly(A)+ sequencing to identify at nucleotide resolution the 3´ ends targeted by the nuclear exosome, and determine the sites of NNS-dependent termination genome-wide.

2016-08-12 | GSE75586 | GEO

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