Project description:To identify potential metastasis associated miRNAs in colorectal cancer (CRC), we performed miRNA array on normal mucosa, CRC tissues without metastasis and CRC tissues with distant metastasis.
Project description:The purpose of this study is to identify miRNAs involved in the pathology of colorectal cancer (CRC) liver metastasis and investigate their underlying mechanisms. A total of 39 miRNAs were identified to be differentially expressed between 16 primary CRC tissues with liver metastases and 16 CRC tissues without liver metastases from 32 patients by Affymetric miRNA microarrays. 16 coloretcal cancer tissues with liver metastasis and 16 colorectal cancer tissues without liver metastasis were included in this study for RNA extraction and hybridization on Affymetrix microarrays. We sought to identify the differentially expressed miRNAs between colorectal cancer tissues with and without liver metastasis.
Project description:Metastasis is a complex process involving multiple steps. We were interested in the role of microRNAs (miRNAs) in the process of liver colonization by colorectal cancer cells. We hypothesized that the comparison between non-metastatic versus metastatic isogenic cell line should thus offer valuable insight to the molecular mechanisms involved in developing metastatic behavior. KM12C/KM12SM and SW480/SW620 are probably the best available models of isogenic cell lines differing in metastatic properties for colorectal cancer. Our first goal was to identify miRNAs that contribute to the metastatic traits of the isogenic colorectal cancer cell lines, KM12C/KM12SM and SW480/SW620. Total RNA was extracted from cells using the mirVana kit (Ambion). Total RNA (1 µg) from KM12C and SW480 (poorly metastatic) and KM12SM and SW620 (highly metastatic) cells was used to analyze the global miRNA expression profiling with TaqMan Megaplex human array A (v2.0) and B (v3.0) (Applied Biosystems).
Project description:The purpose of this study is to identify miRNAs involved in the pathology of colorectal cancer (CRC) liver metastasis and investigate their underlying mechanisms. A total of 39 miRNAs were identified to be differentially expressed between 16 primary CRC tissues with liver metastases and 16 CRC tissues without liver metastases from 32 patients by Affymetric miRNA microarrays.
Project description:Background: Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. The roles of microRNAs (minRNAs) in mRNA destabilization and translational repression of this disease are well appreciated, their involvement in endonucleolytic cleavage of target mRNAs is poorly understood. Methods: High throughput small RNA sequencing was employed for the identification and profiling of miRNAs by six pairs of colorectal cancer tissues (CT) and their adjacent tissues (CN). GO (gene ontology) biological process analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis was carried out to understand the role of regulated miRNAs in CRC. Results: Using high-throughput sequencing, we identified 1409 miRNAs in the CT and CN libraries, including 81 novel miRNAs. 420 conserved and eight novel differentially expressed miRNAs (DEmiRNAs) were identified by comparing the expression levels of CT libraries with CN libraries. Conclusions: Our findings may lead to a better understanding of the novel role of miRNA in the gene regulation of CRC.
Project description:Identification of miRNAs during colorectal cancer progression and metastasis using a preclinical mouse model during LDM topotecan chemotherapy
Project description:The survival rate of patients with colorectal cancer (CRC) declines sharply with recurrence and metastasis. Cancer stem cells play a crucial role in the initiation, progression, recurrence, and metastasis of colorectal cancer. Therefore, in this study, we aimed to identify the CRC-associated genes via spatial analyses of normal colon tissue, colorectal cancer tissue, and colorectal cancer liver metastasis using NanoString GeoMx digital spatial profiling.