Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ectopic expression of the homeobox gene Cdx2 in hematopoietic stem cells

ABSTRACT: Ectopic expression of the homeobox gene Cdx2 in hematopoietic stem cells

PROVIDER: PRJNA493134 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7903602_1.fastq.gz	Fastqsanger.gz
	SRR7903602_2.fastq.gz	Fastqsanger.gz
	SRR7903603_1.fastq.gz	Fastqsanger.gz
	SRR7903603_2.fastq.gz	Fastqsanger.gz
	SRR7903604_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Ectopic expression of the homeobox gene Cdx2 in hematopoietic stem cells

Project description:This dataset describes the transcriptomic profiling by mRNA-seq of the bone marrow of mice either wild type or ectopically expressing the homeobox gene Cdx2 in hematopoietic stem cells. Each condition was analyzed in triplicates to address the role of Cdx2 in leukemogenesis.

2021-07-05 | GSE120487 | GEO

Ectopic expression of the homeobox gene Cdx2 in the epiblast

Project description:Ectopic expression of the homeobox gene Cdx2 in the epiblast

| PRJNA509220 | ENA

Transcription profiling of mouse early endoderm from wild type and homeobox transcription factor Cdx2 knock-out strains

Project description:We demonstrate that conditional ablation of the homeobox transcription factor Cdx2 from early endoderm results in the replacement of the posterior intestinal epithelium with keratinocytes, a dramatic cell fate conversion caused by ectopic activation of the foregut/esophageal differentiation program. This anterior homeotic transformation is first evident in the early embryonic Cdx2-deficient gut as expression of several key foregut endoderm regulators was shifted caudally. While the intestinal transcriptome was severely affected, Cdx2-deficiency only transiently modified selected posterior Hox genes and the primary enteric Hox code was maintained. Further, we demonstrate that Cdx2-directed intestinal cell fate adoption plays an important role in the establishment of normal epithelial-mesenchymal interactions, as multiple signaling pathways involved in this process were severely affected. We conclude that Cdx2 controls important aspects of intestinal identity and development, and that this function is largely independent of the enteric Hox code. Gene ablation was achieved by creating a Cdx2 loxP/loxP mouse which was then crossed with a Foxa3-CRE mouse to yield Cdx2 loxP/loxP Foxa3-CRE mice.

2009-03-19 | E-MTAB-92 | biostudies-arrayexpress

Ectopic expression of the homeobox gene Cdx2 in the epiblast

Project description:This dataset describes the transcriptomic profiling by mRNA-seq of head tissues of E10.5 control or ectopically Cdx2-expressing embryos. Each condition was analyzed in 3 heads to address the role of Cdx2 in embryonic axis determination.

2019-11-04 | GSE123559 | GEO

Hematopoietic stem cell-restricted Cdx2 expression induces transformation to myelodysplasia and acute leukemia [RNASeq_Cdx2_secondaryAML]

Project description:The caudal-related homeobox transcription factor CDX2 is ectopically expressed in the majority of patients with acute myeloid leukemia (AML). We generated an inducible transgenic mouse model whereby Cdx2 was specifically activated in HSCs . Cdx2 mice developed myelodysplastic syndrome (MDS) with progression to acute leukemia associated with stepwise acquisition of additional driver mutations.

2019-09-30 | GSE133828 | GEO

Hematopoietic stem cell-restricted Cdx2 expression induces transformation to myelodysplasia and acute leukemia [RNASeq_Cdx2_4weekspost_Tamoxifen]

Project description:The caudal-related homeobox transcription factor CDX2 is ectopically expressed in the majority of patients with acute myeloid leukemia (AML). We generated an inducible transgenic mouse model whereby Cdx2 was specifically activated in HSCs. Cdx2 mice developed myelodysplastic syndrome (MDS) with progression to acute leukemia associated with stepwise acquisition of additional driver mutations.

2019-09-30 | GSE133679 | GEO

Hematopoietic stem cell-restricted Cdx2 expression induces transformation to myelodysplasia and acute leukemia [Chip-Seq]

Project description:The caudal-related homeobox transcription factor CDX2 is ectopically expressed in the majority of patients with acute myeloid leukemia (AML). We generated an inducible transgenic mouse model whereby Cdx2 was specifically activated in HSCs. Cdx2 mice developed myelodysplastic syndrome (MDS) with progression to acute leukemia associated with stepwise acquisition of additional driver mutations.

2020-03-09 | GSE146598 | GEO

Embryonic stem cells derived from developmentally incompetent embryos deficient in maternal and zygotic Cdx2

Project description:Separation of cell lineages during early mammalian development is required to establish the pluripotent founder cell population that will give rise to the embryo proper and a functional trophoblast to support its development. We systemically assessed the role of the homeobox gene Cdx2 in vivo and in vitro development with an RNAi approach. Effective elimination of both maternal and zygotic Cdx2 resulted in typical phenotypes of Cdx2-mutant embryos, such as failure of hatching and implantation. However, the blastulation and expression of TE specific markers in these Cdx2-deficient embryos excluded the possibility of Cdx2 to act as a TE determinant, although compromised structure and functioning of TE was observed and the resulted embryos were not viable. Strikingly, the efficiency of stem cell derivation was significantly higher than control when embryos were put on MEF at the 8-cell stage and the derived stem cells were fully pluripotent as shown by chimera and tetraploid complementation experiments. Comparative genomic hybridization of wild type and Cdx2 mutant at 8-cell and blastocyst mouse embryos were performed. 8-cell biological duplicates and blastocyst stage biological triplicates embryos were used.The hybridization experiments were duplicated in a reciprocal labeling manner to reduce dye integration bias (dye-swaps).

2008-12-29 | E-GEOD-12330 | biostudies-arrayexpress