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Myxococcus xanthus strain:DK1622

ABSTRACT: Myxococcus xanthus DK1622 transcriptome

PROVIDER: PRJNA493545 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8466770_1.fastq.gz	Fastqsanger.gz
	SRR8466770_2.fastq.gz	Fastqsanger.gz
	SRR8466761_1.fastq.gz	Fastqsanger.gz
	SRR8466761_2.fastq.gz	Fastqsanger.gz
	SRR8466771_1.fastq.gz	Fastqsanger.gz

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Project description:To identify CdbA binding sites on Myxococcus xanthus genome in vivo we performed ChIP-seq, using a polyclonal anti-FLAG antibody and a strain endogenously expressing CdbA_3xFLAG. A WT DK1622 strain was used a negative control and a strain endogenously expressing ParB_3xFLAG was used as positive control.

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Project description:Myxococcus xanthus strain:DK1622::pDPO-Mxn116-Pvan-Tpase | isolate:#1 Genome sequencing

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Project description:In Myxococcus xanthus 55% of the more than 250 two-component signal transduction systems (TCS) genes are orphan. We hypothesized that the histidine kinase SgmT and the response regulator DigR, which comprises a DNA binding domain of the HTH_Xer type, function together to regulate gene expression. We performed genome-wide expression profiling experiments to determine wether the same set of genes are differentially expressed in the ΔdigR and ΔsgmT mutants. 3 biological replicates each; normalized ratios to vegetative cells of DK1622 (wt) Cy5

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