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Genome-wide expression analysis of human hTert immortalized fibroblasts after downregulation of MCM2 & MCM7

ABSTRACT: Genome-wide expression analysis of human hTert immortalized fibroblasts after downregulation of MCM2 & MCM7

PROVIDER: PRJNA494413 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7956090.fastq.gz	Fastqsanger.gz
	SRR7956100.fastq.gz	Fastqsanger.gz
	SRR7956091.fastq.gz	Fastqsanger.gz
	SRR7956101.fastq.gz	Fastqsanger.gz
	SRR7956092.fastq.gz	Fastqsanger.gz

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Genome-wide expression analysis of human hTert immortalized fibroblasts after downregulation of MCM2

Project description:Minichromosome maintenance (MCM) proteins facilitate replication by licensing origins and unwinding the DNA double strand. Interestingly, the number of MCM hexamers greatly exceeds the number of firing origins suggesting additional roles of MCMs. Here we show a hitherto unanticipated function of MCM2 in cilia formation in human cells and zebrafish that is uncoupled from replication. Zebrafish depleted of MCM2 develop ciliopathy-phenotypes including microcephaly and aberrant heart looping due to malformed cilia. In non-cycling human fibroblasts, loss of MCM2 promotes transcription of a subset of genes, which cause cilia shortening and centriole overduplication. Chromatin immunoprecipitation experiments show that MCM2 binds to transcription start sites of cilia inhibiting genes. We propose that such binding may block RNA polymerase II-mediated transcription. Depletion of a second MCM (MCM7), which functions in complex with MCM2 during its canonical functions, reveals an overlapping cilia-deficiency phenotype likely unconnected to replication, although MCM7 appears to regulate a distinct subset of genes and pathways. Our data suggests that MCM2 and 7 exert a role in ciliogenesis in post-mitotic tissues.

2018-10-20 | GSE120766 | GEO

Genome-wide expression analysis of human hTert immortalized fibroblasts after downregulation of MCM2

Project description:Genome-wide expression analysis of human hTert immortalized fibroblasts after downregulation of MCM2

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Genome-wide expression analysis of human hTert immortalized fibroblasts after donwregulation of MCM7

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Genome-wide expression analysis of human hTert immortalized fibroblasts after donwregulation of MCM7

Project description:Genome-wide expression analysis of human hTert immortalized fibroblasts after donwregulation of MCM7

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Comparison of the transcriptomes of hTERT-immortalized and wild type marmoset fibroblasts by next-generation RNA sequencing.

Project description:Neuborn marmoset fibroblasts were immortalized by piggyBac transposition of hTERT expression cassette. The gene expression of the resulting one polyclonal and two monoclonal immortalized cell lines were then compared with the wild type cells used for their derivation.

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Genome-wide expression analysis of human hTert immortalized fibroblasts after downregulation of MCM2 & MCM7

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-10-20 | GSE120768 | GEO

Expression data for hTERT-immortalized human Sertoli cell line versus original primary human Sertoli cells

Project description:Primary human Sertoli cells (hSerCs) were immortalized by transducing human TERT into the genome to produce human TERT-immortalized human Sertoli cells (hTERT hSerCs). Total RNA was isolated from both primary hSerCs and hTERT hSerCs and submitted for Affymetrix gene analysis. Gene expression differences of common Sertoli cell marker genes and xenobiotic transporters was preferentially assessed between each cell type.

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