Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Promoter-proximal pausing mediated by the exon junction complex regulates splicing [RBP1 ChIP]

ABSTRACT: Promoter-proximal pausing mediated by the exon junction complex regulates splicing [RBP1 ChIP]

PROVIDER: PRJNA494822 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7968400.fastq.gz	Fastqsanger.gz
	SRR7968401.fastq.gz	Fastqsanger.gz
	SRR7968402.fastq.gz	Fastqsanger.gz
	SRR7968397.fastq.gz	Fastqsanger.gz
	SRR7968398.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Promoter-proximal pausing mediated by the exon junction complex regulates splicing

Project description:Promoter-proximal pausing of RNA polymerase II (Pol II) is a widespread transcriptional regulatory step across metazoans. Here we find that the nuclear exon junction complex (pre-EJC) is a critical and conserved regulator of this process. Depletion of pre-EJC subunits leads to a global decrease in Pol II pausing and to premature entry into elongation. This effect occurs, at least in part, via non-canonical recruitment of pre-EJC components at promoters. Failure to recruit the pre-EJC at promoters results in increased binding of the positive transcription elongation complex (P-TEFb) and in enhanced Pol II release. Notably, restoring pausing is sufficient to rescue exon skipping and the photoreceptor differentiation defect associated with depletion of pre-EJC components in vivo. We propose that the pre-EJC serves as an early transcriptional checkpoint to prevent premature entry into elongation, ensuring proper recruitment of RNA processing components that are necessary for exon definition.

2019-01-22 | GSE92389 | GEO

Promoter-proximal pausing mediated by the exon junction complex regulates splicing

Project description:Promoter-proximal pausing mediated by the exon junction complex regulates splicing

| PRJNA357409 | ENA

Mnase Sequencing [Promoter-proximal pausing mediated by the exon junction complex regulates splicing]

2019-01-22 | GSE92510 | GEO

Promoter-proximal pausing mediated by the exon junction complex regulates splicing [RBP1 ChIP]

2019-01-22 | GSE120881 | GEO

Promoter-proximal pausing mediated by the exon junction complex regulates splicing [HA ChIP]

2019-01-22 | GSE120880 | GEO

polyA RNA -Seq [Promoter-proximal pausing mediated by the exon junction complex regulates splicing]

2019-01-22 | GSE92388 | GEO