Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Solanum lycopersicum cultivar:Moneymaker

ABSTRACT: transcriptome, sRNAome and methylome of tomatoes infected by tomato yellow leaf curl virus (TYLCV)

PROVIDER: PRJNA494932 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25521604_1.fastq.gz	Fastqsanger.gz
	SRR25521604_2.fastq.gz	Fastqsanger.gz
	SRR25521605_1.fastq.gz	Fastqsanger.gz
	SRR25521605_2.fastq.gz	Fastqsanger.gz
	SRR25521606_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 141

Similar Datasets

Transcriptome profiling of RNAi-trasgenic Solanum lycopersicum plants resistant to Tomato yellow leaf curl virus

Project description:RNA interference (RNAi) is a widely-used approach to generate virus-resistant transgenic crops. However, durability of RNAi-mediated resistance under extreme field conditions and side-effects of stable RNAi expression have not been thoroughly investigated. Here we performed field trials and molecular characterization of two RNAi-transgenic Solanum lycopersicum lines resistant to Tomato yellow leaf curl virus (TYLCV) disease, the major constraint for tomato cultivation in Cuba and worldwide. In order to determine potential impact of the hairpin RNA transgene expression on tomato genome expression and development, differences in the phenotypes and the transcriptome profiles between the transgenic and non-transgenic plants were examined. Transcriptome profiling revealed a common set of up- and down-regulated tomato genes, which correlated with slight developmental abnormalities in both transgenic lines.

2014-11-29 | GSE63708 | GEO

Solanum lycopersicum cultivar:Moneymaker

Project description:Changes of tomato transcriptomes upon Tomato yellow leaf curl virus infection by RNA-Seq

| PRJNA480532 | ENA

Transcriptional reprogramming in transgenic Arabidopsis plants expressing C4G2A and a cas-1 mutant upon activation of plant immunity.

Project description:Transcriptional overlap between transgenic Arabidopsis plants expressing C4G2A from the Tomato yellow leaf curl virus (TYLCV) and a cas-1 mutant upon activation of plant immunity by treatment with the bacterial peptide elicitor flg22 (1 µM, 12 h).

2021-04-12 | GSE148575 | GEO

Identification of tomato proteins that interact with Replication initiator protein (Rep) of the geminivirus TYLCV

Project description:In this study, an GFP-based affinity purification followed by mass spectrometry (AP-MS) approach is used in order to identify new tomato proteins that interact with the Replication initiator protein (Rep, AL1, C1) from Tomato yellow leaf curl virus (TYLCV).

2020-07-10 | PXD018011 | Pride

Solanum lycopersicum cultivar:Moneymaker

Project description:Transcriptome profile analysis of transgenic tomato plants expressing the C4 gene of TYLCV

| PRJNA637749 | ENA

Solanum lycopersicum

Project description:Expression analysis of tomato plants TYLCV resistant, susceptible and resistant line silenced in the hexose transporter (LeHT1) gene before and 7 days after inoculation of tomato with Tomato yellow leaf curl virus (TYLCV).

| PRJNA175901 | ENA

Ethylene receptor protein quantification in Solanum lycopersicum

Project description:Ethylene receptor protein quantification is essential to study their functions, but is impaired by low resolutive tools such as antibodies that are mostly nonspecific. Here we report a proteomic method that enables the quantification of all tomato ethylene receptors, which can be applied to other organisms. Testing this method, we found that “Never-Ripe” tomatoes stay orange while a mutated receptor accumulated at ripening, further blocking the ethylene signal .

2019-10-11 | PXD011412 | Pride

Solanum lycopersicum

Project description:Tomato responses to the whitefly-transmitted TYLCV

| PRJNA101059 | ENA

Genome-wide miRNA and piRNA profiles of the vector whitefly, Bemisia tabaci during feeding on Tomato yellow leaf curl virus-infected tomato

Project description:The whitefly, Bemisia tabaci MEAM1 is a devastating vector capable of transmitting hundreds of plant viruses, including Tomato yellow leaf curl virus (TYLCV), to important food and fiber crops. Here we performed genome-wide profiling of micro RNAs (miRNAs) and piwi-interacting RNAs (piRNAs) in whiteflies after feeding on TYLCV-infected tomato or uninfected tomato for 24, 48 and 72 h. Overall, 160 miRNAs were discovered, 68 of which were conserved and 92 were B. tabaci-specific miRNAs. Majority of the genes that were predicted to be targeted by miRNAs had gene ontologies related to metabolic processes. We identified two miRNAs that were differentially expressed in whiteflies when fed on TYLCV-infected tomato compared to whiteflies that fed on uninfected tomato. The identified piRNAs were expressed as clusters throughout the whitefly genome. A total of 53 piRNA clusters were expressed across all time points and treatments, while 5 piRNA clusters were exclusively expressed in whiteflies that fed on TYLCV-infected tomato, and 24 clusters were exclusively expressed in whiteflies that fed on uninfected tomato. Approximately 62% of all identified piRNAs were derived from non-coding sequences that included intergenic regions, introns, and UTRs with unknown functions. The remaining 38% of piRNAs were derived from coding sequences (CDS) and repeat elements. Transposable elements targeted by piRNA clusters included both class I retrotransposons such as Gypsy, Copia, and LINEs and class II DNA transposons such as MITE, hAT, and TcMar. Lastly, six protein coding genes were targeted in whiteflies that fed on TYLCV-infected tomato. Information on how TYLCV influences miRNA and piRNA expression in whiteflies provides a greater understanding of regulatory pathways involved in mediating whitefly-virus interactions, and will facilitate the identification of novel targets for RNAi control.

2019-02-28 | GSE111343 | GEO

Solanum lycopersicum

Project description:Exome sequencing of tomato curl mutants

| PRJDB5932 | ENA