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Clostridium autoethanogenum strain:H21-9

ABSTRACT: Clostridium autoethanogenum strain:H21-9 Genome sequencing and assembly

PROVIDER: PRJNA495700 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	RFAQ01.dat.gz	Other
	RFAQ01.fasta.gz	Fasta.gz
	RFAQ01.master.dat	Other

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Clostridium autoethanogenum

Project description:Clostridium autoethanogenum overview

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Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness

Project description:Microbes able to convert gaseous one-carbon (C1) waste feedstocks are increasingly important to transition to the sustainable production of renewable chemicals and fuels. Acetogens are interesting biocatalysts since gas fermentation using Clostridium autoethanogenum has been commercialised. However, most acetogen strains need complex nutrients, display slow growth, and are not robust for bioreactor fermentations. In this work, we used three different and independent adaptive laboratory evolution (ALE) strategies to evolve the wild-type C. autoethanogenum to grow faster, without yeast extract and to be robust in operating continuous bioreactor cultures. Multiple evolved strains with improved phenotypes were isolated on minimal media with one strain, named “LAbrini”, exhibiting superior performance regarding the maximum specific growth rate, product profile, and robustness in continuous cultures. Whole-genome sequencing of the evolved strains identified 25 mutations. Of particular interest are two genes that acquired seven different mutations across the three ALE strategies, potentially as a result of convergent evolution. Reverse genetic engineering of mutations in potentially sporulation-related genes CLAU_3129 (spo0A) and CLAU_1957 recovered all three superior features of our ALE strains through triggering significant proteomic rearrangements. This work provides a robust C. autoethanogenum strain “LAbrini” to accelerate phenotyping and genetic engineering and to better understand acetogen metabolism.

2024-06-12 | GSE225123 | GEO

Clostridium autoethanogenum DSM 10061

Project description:Clostridium autoethanogenum DSM 10061 Genome sequencing and assembly

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Clostridium autoethanogenum DSM 10061

Project description:Clostridium autoethanogenum DSM 10061 Genome sequencing and assembly

| PRJNA291963 | ENA

Clostridium autoethanogenum isopropanol production via native plasmid pCA replicon

Project description:Clostridium autoethanogenum isopropanol production via native plasmid pCA replicon

2022-05-10 | MSV000089452 | MassIVE

Clostridium autoethanogenum DSM 10061

Project description:Clostridium autoethanogenum DSM 10061 Genome sequencing

| PRJNA219420 | ENA

Systems-level engineering and characterization of Clostridium autoethanogenum through heterologous production of poly-3-hydroxybutyrate (PHB)

Project description:Gas fermentation is emerging as an economically attractive option for the sustainable production of fuels and chemicals from gaseous waste feedstocks. Clostridium autoethanogenum can use CO and/or CO2 + H2 as its sole carbon and energy sources. Fermentation of C. autoethanogenum is currently being deployed on a commercial scale for ethanol production. Expanding the product spectrum of acetogens will enhance the economics of gas fermentation. To achieve efficient heterologous product synthesis, limitations in redox and energy metabolism must be overcome. Here, we engineered and characterised at a systems-level, a recombinant poly-3-hydroxybutyrate (PHB)-producing strain of C. autoethanogenum. Cells were grown in CO-limited steady-state chemostats on two gas mixtures, one resembling syngas (20% H2) and the other steel mill off-gas (2% H2). Results were characterised using metabolomics and transcriptomics, and then integrated using a genome-scale metabolic model reconstruction. PHB-producing cells had an increased expression of the Rnf complex, suggesting energy limitations for heterologous production. Subsequent optimisation of the bioprocess led to a 12-fold increase in the cellular PHB content. The data suggest that the cellular redox state, rather than the acetyl-CoA pool, was limiting PHB production. Integration of the data into the genome-scale metabolic model showed that ATP availability limits PHB production. Altogether, the data presented here advances the fundamental understanding of heterologous product synthesis in gas-fermenting acetogens.

2019-02-03 | GSE115837 | GEO

Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness

2024-06-12 | PXD047330 | Pride

Clostridium autoethanogenum strain:Lab Adapted Abrini | isolate:S232868

Project description:Clostridium autoethanogenum strain:Lab Adapted Abrini | isolate:S232868 Genome sequencing

| PRJNA896243 | ENA

Effect of visible light on the transcriptome of Clostridium autoethanogenum during gas fermentation

Project description:The expression profile of C. autoethanogenum DSM 10061 grown autotrophically with H2:CO:CO2 under visible light at an intensity of 4200 lux versus the expression profile of C. autoethanogenum DSM 10061 grown autotrophically in the dark

2023-07-01 | GSE207468 | GEO

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