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Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo (RNA-Seq)

ABSTRACT: Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo (RNA-Seq)

PROVIDER: PRJNA496417 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8054040.fastq.gz	Fastqsanger.gz
	SRR8054050.fastq.gz	Fastqsanger.gz
	SRR8054041.fastq.gz	Fastqsanger.gz
	SRR8054051.fastq.gz	Fastqsanger.gz
	SRR8054042.fastq.gz	Fastqsanger.gz

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Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo

Project description:Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo

| PRJNA516453 | ENA

Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo

Project description:A long-prevailing model has held that the “seed” region (nucleotides 2-8) of a microRNA is typically sufficient to mediate target recognition and repression. However, numerous recent studies, both within the context of defining miRNA/target pairs by direct physical association and by directly assessing this model in vivo in C. elegans have brought this model into question. To test the importance of miRNA 3' pairing in vivo, in a mammalian system, we engineered a mutant murine mir-146a allele in which the 5' half of the mature microRNA retains the sequence of the wild-type mir-146a but the 3ʹ half has been altered to be anti-complementary to the wild-type miR-146a sequence. Mice homozygous or hemizygous for this mutant allele are phenotypically indistinguishable from wild-type controls and do not recapitulate any of the immunopathology previously described for mir-146a-null mice. Our results strongly support the conclusion that 3ʹ pairing is dispensable in the context of the function of a key mammalian microRNA.

2019-02-09 | PXD011413 | Pride

Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo (miRNA-Seq)

Project description:Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo (miRNA-Seq)

| PRJNA516456 | ENA

Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo (RNA-Seq)

2019-02-06 | GSE121232 | GEO

Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo (miRNA-Seq)

2019-02-06 | GSE125473 | GEO

MiR-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo.

Project description:The prevailing model of microRNA function is that the "seed region" (nt 2-8) is sufficient to mediate target recognition and repression. However, numerous recent studies have challenged this model, either by demonstrating extensive 3' pairing between physically defined miRNA-mRNA pairs or by showing in Caenorhabditis elegans that disrupted 3' pairing can result in impaired function in vivo. To test the importance of miRNA 3' pairing in a mammalian system in vivo, we engineered a mutant murine mir-146a allele in which the 5' half of the mature microRNA retains its wild-type sequence, but the 3' half's sequence has been altered to robustly disrupt predicted pairing to this latter region. Mice homozygous or hemizygous for this mutant allele are phenotypically indistinguishable from wild-type controls and do not recapitulate any of the immunopathology previously described for mir-146a-null mice. Our results indicate that 3' pairing is dispensable for the established myeloid function of this key mammalian microRNA.

| S-EPMC6379685 | biostudies-literature

Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-02-06 | GSE125474 | GEO

Data in support of Absence of miRNA-146a differentially alters microglia function and proteome

Project description:MiR-146a is an important regulator of innate inflammatory responses and is also implicated in cell death and survival. Here, we identified microglia as the main cellular source of miR-146a among mouse CNS resident cells. We further characterized the phenotype of miR-146a KO microglia cells during in vivo demyelination induced by cuprizone (CPZ) and found reduced number of CD11c+ microglia in the KO compared to WT mice. Microglia were also isolated from the brain, and the proteome was analyzed by liquid chromatography mass spectrometry.

2020-06-29 | PXD015939 | Pride

Understanding transcriptional identity in Innate Lymphoid Cells (ILC)

Project description:Understanding transcriptional identity in Innate Lymphoid Cells (ILC)

| PRJEB41498 | ENA

miR-146a regulates many lncRNAs in RAS induced senescence [Anti-146a]

Project description:miR-146a is a known anti-inflammatory miRNA. Intringuigly, it is overexpressed in RAS-induced senescent cells which is accompanied with a rich pro-inflammatoy secretpry phenotype. We aim to study possible sponges for miR-146a.

2021-05-11 | GSE150325 | GEO

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