Project description:Germ-free wild-type mice (GFWT) were inoculated with the commensal CEC (CECWT) or probiotic Nissle 1917 (NissleWT) E. coli strain. Mono-colonized mice, CECWT and NissleWT, were sacrificed 21 days post-inoculation and the colonic gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays.
Project description:Germ-free wild-type mice (GFWT) were inoculated with the commensal CEC (CECWT) or probiotic Nissle 1917 (NissleWT) E. coli strain. Mono-colonized mice, CECWT and NissleWT, were sacrificed 21 days post-inoculation and the ileal and colonic gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays.
Project description:Intrarectally DNBS-treated groups, to provoke colitis, were treated orally with the commensal CEC (CEC) or probiotic Nissle 1917 (Nissle) E. coli strains. Healthy control group was treated intrarectally and orally with PBS (PP). Sick control group was treated intrarectally with DNBS and orally with PBS (DP). Mice were sacrificed 24 days post first DNBS injection and the colonic gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays Mouse Inflammation Panel.
Project description:Identification of altered blood microRNAs in a rat model of Parkinson’s disease by Custom "Brain Specific miRNA" Open Array Real-time PCRpanel
Project description:Intrarectally DNBS-treated groups, to provoke colitis, were treated orally with the commensal CEC (CEC) or probiotic Nissle 1917 (Nissle) E. coli strains. Healthy control group was treated intrarectally and orally with PBS (PP). Sick control group was treated intrarectally with DNBS and orally with PBS (DP). Mice were sacrificed 24 days post first DNBS injection and the ileal gene expression profiles analyzed by high-throughput qPCR using TaqMan OpenArrays Mouse Inflammation Panel.
Project description:The purpose of this study was to investigate the relative mRNA expression related to hormone, antioxidant capacity and immune responses in jejunal and ileal mucosa of healthy and postnatal growth retardation pigs. At 42 d of age, after overnight fasting, six postnatal growth retardation pigs and six healthy pigs were pair-matched by litter were selected for sampling. Samples of the jejunal and ileal mucosa were scraped and immediately snap-frozen in liquid nitrogen and stored at −80°C for RNA extraction. We used Roche LightCycler ® 480 Instrument PCR assay panel to quantitate gene expression of hormone, antioxidant capacity and immune responses relevant genes from jejunal and ileal mucosa.