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Equus asinus breed:Donkey

ABSTRACT: Equus asinus breed:Donkey Transcriptome or Gene expression

PROVIDER: PRJNA498718 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8116690.fastq.gz	Fastqsanger.gz
	SRR8116691_1.fastq.gz	Fastqsanger.gz
	SRR8116691_2.fastq.gz	Fastqsanger.gz
	SRR8116692_1.fastq.gz	Fastqsanger.gz
	SRR8116692_2.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of Sertoli cells in Equus asinus

Project description:Deoxynivalenol (DON) and zearalenone (ZEA) are the most common mycotoxins, which are damaging to the reproductive system of humans and animals. Sertoli cells perform vital roles of male reproduction include composition of the blood testis barrier, support and release sperm. In the present study, transcriptomic analysis was conducted on different dosages (0 μM, 10 μM and 30 μM) of DON and ZEA treated donkey Sertoli cells. Transcriptomic analysis showed 6551 differentially expressed genes (DEGs) were identified between DON 10 μM treatment group and control group, with 3300 up regulated and 3251 down; 8605 DEGs were identified between DON 30 μM treatment group and control group, with 4841 up regulated and 3764 down; 803 DEGs were identified between ZEA 10 μM treatment group and control group, with 391 up regulated and 412 down; and 5947 DEGs were identified between ZEA 30 μM treatment group and control group, with 2816 up regulated and 3131 down. In DEGs, Caspase-1, GSDMD-NT, ESR 1, AR, PRDX 4, NOX 1, MSH 6 and CDK 1, were expressed highly in DON and ZEA treatment group, which related to pyroptosis, hormone secretion, oxidative stress and cancerous. It is unprecedented that DON induced cell death by pyroptosis. This study provides valuable genetic resources for understanding the mechanisms and pathways of the toxin effect of DON and ZEA for Equus asinus' Sertoli cells.

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