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Pleurotus tuoliensis

ABSTRACT: Pleurotus tuoliensis overview

PROVIDER: PRJNA498729 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR21986371_1.fastq.gz	Fastqsanger.gz
	SRR21986371_2.fastq.gz	Fastqsanger.gz
	SRR21986372_1.fastq.gz	Fastqsanger.gz
	SRR21986372_2.fastq.gz	Fastqsanger.gz

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A comparative transcriptome analysis reveals physiological maturation properties of mycelia in Pleurotus tuoliensis

Project description:Pleurotus tuoliensis is a precious edible fungus with extremely high nutritive and medicinal value. The cultivation period of P. tuoliensis is longer than those of other Pleurotus species, which is mainly due to a longer mycelium physiological maturation period (30-60 days). Currently, the molecular processes underlying physiological maturation of the mycelium remain unclear. We performed a comparative transcriptomic analysis of immature and mature mycelia using RNA-seq. De novo transcriptome assembly resulted in identification of 17,030 unigenes. 451 differentially expressed genes, including those encoding nucleoside diphosphate kinase (NDPK), glycoside hydrolase family proteins, exopolygalacturonase, and versatile peroxidases, were identified. GO and KEGG analyses revealed that nucleotide synthesis and energy metabolism are highly active during the physiological maturation of mycelia, and genes related to these pathways were significantly up-regulated in mature mycelia. NDPK is predicted to be essential for mycelia maturation. Our findings contribute to a comprehensive understanding of mycelia maturation in a commercially important fungal species. Future efforts will focus on the function of NDPK and the mechanism by which it regulates mycelia maturation.

2019-08-20 | GSE135839 | GEO

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Comparative analysis of genome-wide transcriptional responses to continuous heat stress in Pleurotus tuoliensis

Project description:The whole-genome gene expression profiling was performed by RNA-Seq after 96 h HS treatment of P. tuoliensis mycelia. Divergent mycelial growth rates were observed under the HS with different intensities, which facilitated comparative analyses combining both physiological and transcriptomic data. The goal aimed to acquire a deeper insight into the genome-wide gene expression patterns during the response to HS in mushrooms.

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