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PolyA RNA Sequencing Analysis of HTR-8/SVneo cells after lnc-SLC4A1-1 overexpression

ABSTRACT: polyA RNA Sequencing Analysis of HTR-8/SVneo cells after lnc-SLC4A1-1 overexpression

PROVIDER: PRJNA499120 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8131620.fastq.gz	Fastqsanger.gz
	SRR8131621.fastq.gz	Fastqsanger.gz
	SRR8131622.fastq.gz	Fastqsanger.gz
	SRR8131623.fastq.gz	Fastqsanger.gz
	SRR8131624.fastq.gz	Fastqsanger.gz

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polyA RNA Sequencing Analysis of HTR-8/SVneo cells after lnc-SLC4A1-1 overexpression

Project description:To further evaluate the functional role of lnc- SLC4A1-1, we overexpressed lnc-SLC4A1-1 in HTR-8/SVneo cells and isolated RNA for RNA-seq. RNA-seq was done using TruSeq Stranded mRNA Library Preparation. Briefly, intact RNA was fragmented, end repaired, adapter ligation and PCR amplified following illumina protocol. Libraries were sequencing by illumins Hiseq 3000. After quality control, sequence data were processed with STAR to generate read alignments with hg19. Raw read counts for annotated genes were obtained with featureCounts with default settings, normalized and analyzed using DEGseq.

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Differential expression of miRNAs in EGF treated HTR-8/SVneo versus untreated control by next generation sequencing

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Differential expression of miRNAs in HGF treated HTR-8/SVneo versus untreated control by next generation sequencing

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Differential expression of mRNAs in IFN-gamma treated HTR-8/SVneo versus untreated control by next generation sequencing

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Project description:The goal of this study is to compare miRNAs expressed by IFN-gamma treated HTR-8/SVneo cells to miRNAs expressed in untreated control HTR-8/SVneo cells to identify micro RNAs which play a role during IFN-gamma-mediated HTR-8/SVneo cells invasion

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Effect of IL-11 on gene expression in JEG-3 and HTR-8/SVneo trophoblastic cells

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