Project description:Illumina HiSeq technology was used to generate mRNA profiles from Suillus luteus ectomycorrhizal roots compared to free-living mycelium . Mycorrhizal roots were harvested after 40 days, pooled and used for RNA extraction. Reads of 2X100bp were generated and aligned to Suillus luteus (http://genome.jgi-psf.org/Suilu1/Suilu1.home.html) using CLC Genomics Workbench 6.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Suillus luteus ectomycorrhizal roots compared to free-living mycelium . Mycorrhizal roots were harvested after 40 days, pooled and used for RNA extraction. Reads of 2X100bp were generated and aligned to Suillus luteus (http://genome.jgi-psf.org/Suilu1/Suilu1.home.html) using CLC Genomics Workbench 6. mRNA profiles from Suillus luteus ectomycorrhizal roots and free-living mycelium were generated by paired-end (2x100bp) Illumina HiSeq2000 sequencing. Two biological replicates were sequenced for mycorrhizal and mycelium samples.
Project description:The antennal transcriptome analysis was performed for F. occidentalis and F. intonsa, and the putative chemosensory genes (OBPs and CSPs) were identified and compared between the two species
Project description:Frankliniella occidentalis is an important insect carrier of virus disease that harms cash crops, and it often causes virus disease to spread widely in cash crops. Post-translational modifications of proteins play an important role in regulating life activities, and the study of newly discovered modifications (PTMs) is of great significance. Lysine Klac (Kla) is an evolutionary conservative form of post-translational modification of a new type of protein discovered in mammals, plants, yeast and pathogenic bacteria in recent years. The study of this modification can increase our understanding of Frankliniella occidentalis, help further study the biological function mechanism of Frankliniella occidentalis and the key to disease transmission, and is of great significance to disease prevention and control.