Project description:In this study we performed single-cell sequencing of E12.5 mouse diencephalon revealing different newly generated neurons and their associated molecular features.
Project description:In our study, we performed single-cell sequencing of the E12.5 mouse placenta to explore the cellular diversity and gene expression dynamics during placental development. This approach allowed us to delineate the complex landscape of cell types and states present at this critical stage of gestation. By analyzing the transcriptomic data, we identified several distinct trophoblast subpopulations, each characterized by unique gene expression patterns that suggest different functional roles in placental structure and function. Additionally, our data provided insights into the potential regulatory pathways involved in cell differentiation.
Project description:The mediodorsal thalamus and adjacent midline nuclei are critical for higher-order cognition and neuropsychiatric disease, yet their cellular organization remains incompletely defined. The objective of this study was to characterize the cellular composition and transcriptional organization of the mouse medial thalamus. Single-nucleus RNA sequencing was used to identify neuronal cell types, assign transcriptional clusters to anatomical nuclei, and define gene expression patterns underlying thalamic organization. A further goal was to compare these features across species to identify conserved transcriptional patterns and differences in cell type composition, including neuronal populations expanded in humans relative to mice. This dataset provides a transcriptomic atlas of the medial thalamus and a resource for investigating cell type diversity, functional organization, and cross-species specialization.
Project description:Thalamus is a brain area where most intense inflammation is observed after cortical brain injuries. Using mice as model, we have examined the gene expression changes of cells in the thalamus after cortical brain damage at the single-nucleus level.
Project description:Knowledge of the full repertoire of thalamus cells and their gene expression profiles is a fundamental first step in this endeavor. Here, using single-nuclei RNA sequencing (snRNA-seq), we sequenced the transcriptomes of 32332 single brain cells, revealing a total of four major cell types within the four thalamus sample from mice.