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Caenibius tardaugens NBRC 16725 isolate:ARI-1

ABSTRACT: High quality whole genome sequence of the estradiol degrader strain Novosphingobium tardaugens NBRC 16725

PROVIDER: PRJNA505257 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR8271516_subreads.fastq.gz	Fastqsanger.gz

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Caenibius tardaugens NBRC 16725

Project description:Novosphingobium tardaugens NBRC 16725 genome sequencing project

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Project description:Whole transcriptomic analysis of Novosphingobium tardaugens NBRC 16725 grown in estradiol raw sequence reads

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Project description:Whole transcriptomic analysis of Novosphingobium tardaugens NBRC 16725 grown in pyruvate raw sequence reads

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Project description:Whole transcriptomic analysis of Novosphingobium tardaugens NBRC 16725 grown in testosterone raw sequence reads

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Project description:High quality whole genome sequence of Novosphingobium tardaugens NBRC 16725

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Project description:Novosphingobium tardaugens overview

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Whole transcriptomic analysis of Novosphingobium tardaugens NBRC 16725 grown in estradiol raw sequence reads

Project description:Whole transcriptomic analysis of Novosphingobium tardaugens NBRC 16725 grown in estradiol raw sequence reads

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Identification of the EdcR Estrogen-Dependent Repressor in Caenibius tardaugens NBRC 16725: Construction of a Cellular Estradiol Biosensor.

Project description:In this work, Caenibius tardaugens NBRC 16725 (strain ARI-1) (formerly Novosphingobium tardaugens) was isolated due to its capacity to mineralize estrogenic endocrine disruptors. Its genome encodes the edc genes cluster responsible for the degradation of 17β-estradiol, consisting of two putative operons (OpA and OpB) encoding the enzymes of the upper degradation pathway. Inside the edc cluster, we identified the edcR gene encoding a TetR-like protein. Genetic studies carried out with C. tardaugens mutants demonstrated that EdcR represses the promoters that control the expression of the two operons. These genetic analyses have also shown that 17β-estradiol and estrone, the second intermediate of the degradation pathway, are the true effectors of EdcR. This regulatory system has been heterologously expressed in Escherichia coli, foreseeing its use to detect estrogens in environmental samples. Genome comparisons have identified a similar regulatory system in the edc cluster of Altererythrobacter estronivorus MHB5, suggesting that this regulatory arrangement has been horizontally transferred to other bacteria.

| S-EPMC8700777 | biostudies-literature

Polyhydroxyalkanoate Production by Caenibius tardaugens from Steroidal Endocrine Disruptors.

Project description:The α-proteobacterium Caenibius tardaugens can use estrogens and androgens as the sole carbon source. These compounds are steroidal endocrine disruptors that are found contaminating soil and aquatic ecosystems. Here, we show that C. tardaugens, which has been considered as a valuable biocatalyst for aerobic steroidal hormone decontamination, is also able to produce polyhydroxyalkanoates (PHA), biodegradable and biocompatible polyesters of increasing biotechnological interest as a sustainable alternative to classical oil-derived polymers. Steroid catabolism yields a significant amount of propionyl-CoA that is metabolically directed towards PHA production through condensation into 3-ketovaleryl-CoA, rendering a PHA rich in 3-hydroxyvalerate. To the best of our knowledge, this is the first report where PHAs are produced from steroids as carbon sources.

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Unraveling the 17β-Estradiol Degradation Pathway in Novosphingobium tardaugens NBRC 16725.

Project description:We have analyzed the catabolism of estrogens in Novosphingobium tardaugens NBRC 16725, which is able to use endocrine disruptors such as 17β-estradiol, estrone, and estriol as sole carbon and energy sources. A transcriptomic analysis enabled the identification of a cluster of catabolic genes (edc cluster) organized in two divergent operons that are involved in estrogen degradation. We have developed genetic tools for this estrogen-degrading bacterium, allowing us to delete by site-directed mutagenesis some of the genes of the edc cluster and complement them by using expression plasmids to better characterize their precise role in the estrogen catabolism. Based on these results, a catabolic pathway is proposed. The first enzyme of the pathway (17β-hydroxysteroid dehydrogenase) used to transform 17β-estradiol into estrone is encoded out of the cluster. A CYP450 encoded by the edcA gene performs the second metabolic step, i.e., the 4-hydroxylation of estrone in this strain. The edcB gene encodes a 4-hydroxyestrone-4,5-dioxygenase that opens ring A after 4-hydroxylation. The initial steps of the catabolism of estrogens and cholate proceed through different pathways. However, the degradation of estrogens converges with the degradation of testosterone in the final steps of the lower catabolic pathway used to degrade the common intermediate 3aα-H-4α(3'-propanoate)7a-β-methylhexahydro-1,5-indanedione (HIP). The TonB-dependent receptor protein EdcT appears to be involved in estrogen uptake, being the first time that this kind of proteins has been involved in steroid transport.

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