Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Metagenome

ABSTRACT: De-constructed Polymerase Chain Reaction (DePCR) as a novel methodology to tackle PCR Bias

PROVIDER: PRJNA506229 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8217620_1.fastq.gz	Fastqsanger.gz
	SRR8217620_2.fastq.gz	Fastqsanger.gz
	SRR8217630_1.fastq.gz	Fastqsanger.gz
	SRR8217630_2.fastq.gz	Fastqsanger.gz
	SRR8217640_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 290

Similar Datasets

The Detection of Circulating Tumor Cells (CTCs) in Patients With Colorectal Cancer Undergoing Cryosurgery Combined With DC-CIK Treatment

Project description:Circulating tumor cells (CTCs) have the potential to provide a surrogate for’real-time biopsy’ of tumor biological activity. Enumeration and molecular characterization of CTCs in colorectal cancer could play an important role in diagnosis, predicting the risk for tumor recurrence, and providing novel target therapy biomarkers. In view of these facts, the investigators wanted to demonstrate the value of multiparameter flow cytometry in detecting human tumor cells of colorectal cancer in normal peripheral blood after cryosurgery with or without dendritic cell(DC)-cytokine-induced killers(CIK) treatment, and the investigators also compared the specificity with reverse transcriptase polymerase chain reaction (RT-PCR) method.

| 2190499 | ecrin-mdr-crc

Whole eye transcriptome generated in Abca4-/-Rdh8-/- mice to identify changes in gene expression after light exposure

Project description:RNA-Seq has revolutionized transcriptome profiling with precise identification of even low expressing and novel transcripts. Goal of the current study was to identify novel gene signatures/biological pathways which are expressed in the early stage after light exposure in our disease model to identify transcriptional mis-regulation followed by validation using quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods

2016-12-16 | GSE75470 | GEO

Revealing the underlying regulatory mechanism of LINC00313 in Osimertinib-resistant NSCLC cells by ceRNA network analysis

Project description:In this study, RNA-sequencing technology was employed to identify the differentially expressed lncRNAs (DE-lncRNAs) and mRNAs (DE-mRNAs) between H1975 and H1975OR. Based on the starbase 2.0 database, the potential targeting relationships between DE-lncRNAs and DE-mRNAs were predicted and identified to construct the ceRNA networks. Subsequently, functional and pathway enrichment analysis were carried for target DE-mRNAs to obtain functional pathways associated with Osimertinib resistance and potential drug resistance-related DE-mRNAs. Besides, the expression of LINC00313 and COL1A1 was validated by q-Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). Finally, the activation of PI3K/Akt pathway was proved by immunohistochemistry staining.

2023-03-31 | GSE228049 | GEO

RNA-sequencing in liver tissues for Wild or Ndrg3-deleted C57BL/6 mice

Project description:NGS-derived liver transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis

2022-05-18 | GSE196110 | GEO

Total RNA transcriptome analysis of hypoxia with sgSETDB1 in hela cells

Project description:Purpose: The goals of this study are to compare NGS-derived Hela with sgSETDB1 and shcontrol in hypoxia transcriptome profiling (RNA-seq) to quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods

2023-11-15 | GSE179329 | GEO

mRNA transcriptome analysis of hypoxia with shSETDB1 in hela cells

Project description:Purpose: The goals of this study are to compare NGS-derived Hela with shSETDB1 and shcontrol in hypoxia transcriptome profiling (RNA-seq) to quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods

2023-11-15 | GSE179327 | GEO

ena-DATASET-THE UNIVERSITY OF HONG KONG DEPARTMENT OF PATHOLOGY AND PFIZER INC.-18-12-2019-11:12:21:554-1297 - samples

Project description:Four micrograms of total RNA was used for cDNA library construction using the KAPA Stranded mRNA-Seq Kit (KR0960-v3.15), following manufacturer's protocol. The adaptor-ligated libraries were enriched by 6 cycles of polymerase chain reaction (PCR). Libraries were sequenced using the Novaseq 6000 with paired end 151bp reads.

| EGAD00001005753 | EGA

RNA-seq analysis of 2D- and 3D-cultured pancreatic cancer cells

Project description:The aim of this study is to evaluate data by comparing transcriptome profiling (RNA-seq) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) methods of pancreatic cancer cells grown in 2D and 3D- culture environments.

2022-03-14 | GSE198192 | GEO

Quantitative reverse transcription PCR analysis of human breast tumor cells in the PAM50 genes

Project description:Human formalin-fixed paraffin-embedded breast tumor tissue (1-4 1.5mm punches) was processed to RNA using High Pure RNA Paraffin Kit (Roche Applied Science, Indianapolis, IN) for quantitative reverse transcription polymerase chain reaction (qRT-PCR).

2018-04-01 | GSE112063 | GEO

RNA Sequencing Facilitates Quantitative Analysis of Albumin-Cre (Control, Ctl) and Liver-specific Klf15 Knockout (Li-KO) Mouse Liver Transcriptomes

Project description:The goals of this study are to compare control and liver Klf15 deficient mouse liver transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis

2019-02-22 | GSE126762 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data