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Spo11-oligo mapping of double-strand breaks in pch2 and sir2 mutants

ABSTRACT: Spo11-oligo mapping of double-strand breaks in pch2 and sir2 mutants

PROVIDER: PRJNA506787 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8240430.fastq.gz	Fastqsanger.gz
	SRR8240431.fastq.gz	Fastqsanger.gz
	SRR8240432.fastq.gz	Fastqsanger.gz
	SRR8240429.fastq.gz	Fastqsanger.gz

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Similar Datasets

Spo11-oligo mapping of double-strand breaks in pch2 and sir2 mutants

Project description:The Spo11-generated double-strand breaks (DSBs) that initiate meiotic recombination are non-randomly distributed across the genome. Here, we use Spo11-oligonucleotide complexes, a byproduct of DSB formation, to map the distribution of meiotic DSBs in pch2 and sir2 mutant strains of Saccharomyces cerevisiae.

2018-11-25 | GSE122882 | GEO

Spo11-oligo mapping of double-strand breaks in spo11 mutants

Project description:Spo11-oligo mapping of double-strand breaks in spo11 mutants

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Spo11-oligo mapping of double-strand breaks in spo11 mutants

Project description:The Spo11-generated double-strand breaks (DSBs) that initiate meiotic recombination are non-randomly distributed across the genome. Here, we use Spo11-oligonucleotide complexes to map the distribution of meiotic DSBs in a spo11 mutant strain of Saccharomyces cerevisiae.

2020-05-12 | GSE150314 | GEO

Double-strand breaks in spo11 mutants

Project description:Double-strand breaks in spo11 mutants

| PRJNA631751 | ENA

Genome-wide mapping of meiotic double-strand breaks by sequencing Spo11 oligos.

Project description:The nonrandom distribution of meiotic recombination shapes patterns of inheritance and genome evolution, but chromosomal features governing this distribution are poorly understood. Formation of the DNA double-strand breaks (DSBs) that initiate recombination results in accumulation of Spo11 protein covalently bound to small DNA fragments. We show here that sequencing these fragments provides a genome-wide DSB map of unprecedented resolution and sensitivity. We use this map to explore the influence of large-scale chromosome structures, chromatin, transcription factors, and local sequence composition on DSB distributions. Our analysis supports the view that the recombination terrain is molded by combinatorial and hierarchical interaction of factors that work on widely different size scales. Mechanistic aspects of DSB formation and early processing steps are also uncovered. This map illuminates the occurrence of DSBs in repetitive DNA elements, repair of which can lead to chromosomal rearrangements. We discuss implications for evolutionary dynamics of recombination hotspots. We deep sequenced 4 samples of Spo11 oligos on Roche 454 platform. Three samples are technical replicates of Spo11 oligo products prepared from one meiotic culture and the fourth sample was prepared from an independent culture.

2011-03-07 | E-GEOD-26449 | biostudies-arrayexpress

Spo11-oligo mapping in S. cerevisiae strain SK1

Project description:Spo11-oligo mapping in S. cerevisiae strain SK1

2020-07-23 | GSE152957 | GEO

Meiotic double-strand break mapping in spo11 mutants by S1Seq

Project description:The Spo11-generated double-strand breaks (DSBs) that initiate meiotic recombination are non-randomly distributed across the genome. Here, we use S1Seq mapping to map the distribution of meiotic DSBs in spo11 mutant strains of Saccharomyces cerevisiae.

2020-05-12 | GSE150313 | GEO

Spo11-oligo mapping in S. cerevisiae strain SK1

Project description:Spo11-oligo mapping in S. cerevisiae strain SK1

| PRJNA641078 | ENA

Spo11-oligo mapping of DSBs in an SK1 x S288C F1 hybrid strain

Project description:The Spo11-generated double-strand breaks (DSBs) that initiate meiotic recombination are non-randomly distributed across the genome. Here, we use Spo11-oligonucleotide complexes, a byproduct of DSB formation, to map the distribution of meiotic DSBs in an SK1 x S88C F1 hybrid strain of Saccharomyces cerevisiae.

2017-07-13 | GSE101339 | GEO

Reconstitution of SPO11-dependent double-strand break formation

Project description:Homologous meiotic recombination starts with DNA double-strand breaks (DSBs) generated by SPO11 protein. SPO11 is critical for meiosis in most species but the DSBs it makes are also dangerous because of their mutagenic and gametocidal potential, so cells must foster beneficial functions of SPO11 while minimizing its risks. SPO11 mechanism and regulation remain poorly understood. Here we report reconstitution of DNA cleavage in vitro with purified recombinant mouse SPO11 bound to its essential partner TOP6BL. Similar to their yeast orthologs, SPO11–TOP6BL complexes are monomeric (1:1) in solution and bind tightly to DNA. Unlike in yeast, however, dimeric (2:2) assemblies of mouse SPO11–TOP6BL cleaves DNA to form covalent 5 prime attachments requiring SPO11 active site residues, divalent metal ions, and SPO11 dimerization. Surprisingly, SPO11 can also manifest topoisomerase activity by relaxing supercoils and resealing DNA that it has nicked. Structure modeling with AlphaFold3 illuminates the protein-DNA interface and suggests that DNA is bent prior to cleavage. Deep sequencing of in vitro cleavage products reveals a rotationally symmetric base composition bias that partially explains DSB site preferences in vivo. Cleavage is inefficient on complex DNA substrates, partly because SPO11 is readily trapped in DSB-incompetent (presumably monomeric) binding states that exchange slowly. However, cleavage is improved by using substrates that favor DSB-competent dimer assembly, or by fusing SPO11 to an artificial dimerization module. Our results inform a model in which intrinsically feeble dimerization restrains SPO11 activity in vivo, making it exquisitely dependent on accessory proteins that focus and control DSB formation so that it happens only at the right time and the right places.

2024-08-20 | GSE275291 | GEO

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