Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell sequencing of mouse heart immune infiltrate in pressure overload-driven heart failure reveals extent of immune activation

ABSTRACT: Single cell sequencing of mouse heart immune infiltrate in pressure overload-driven heart failure reveals extent of immune activation

PROVIDER: PRJNA506965 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8245100.fastq.gz	Fastqsanger.gz
	SRR8245110.fastq.gz	Fastqsanger.gz
	SRR8245120.fastq.gz	Fastqsanger.gz
	SRR8245101.fastq.gz	Fastqsanger.gz
	SRR8245111.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 28

Similar Datasets

Single cell sequencing of mouse heart immune infiltrate in pressure overload-driven heart failure reveals extent of immune activation

Project description:Single-cell RNA sequencing is revolutionizing the mapping of tissues in physiology and pathology. Whilst immune responses are emerging as key mediators of heart failure, no thorough immune mapping of the ailing heart has been performed so far. Here we examined, at single-cell level, the cardiac immune composition after transverse aortic constriction, the standard murine model of non-ischemic heart failure. We identified the presence of most major immune cell subpopulations in the myocardium of both healthy and cardiopathic mice. Despite the absence of infectious agents or an autoimmune trigger, the ailing heart, especially at late-stage disease, is characterized by an immune activation that is both drastic and far more wide-ranging in the immune cell types involved than previously thought. The molecular-level detail we uncover in the cells undergoing activation in response to cardiac stress may enable the identification of novel biomarkers and therapeutic targets for heart failure.

2019-10-12 | GSE122930 | GEO

Single cell transcriptomics of cardiac immune cells after pressure overload

Project description:Cardiac immune cells play important roles in orchestrating the response of the heart to stress and injury. The precise role of immune cells in the early phase after cardiac pressure overload is unclear. Clustering analysis of >33,000 sequenced single cells identified 25 immune cell populations including 12 macrophage and monocyte populations.

2021-08-01 | GSE179276 | GEO

Transcription profiling of mouse heart samples from pressure overload and sham treated animals from wild type and Apelin knockouts reveals impaired heart function in Apelin

Project description:The endogenous peptide Apelin is crucial for maintaining heart function in pressure overload and aging Experiment Overall Design: Heart samples from Apelin knockout mice with pressure overload and sham control together with the wild-type mice with pressure overload and sham were compared

2008-06-15 | E-GEOD-7781 | biostudies-arrayexpress

expression data from Zbtb17 cKO mouse heart after pressure overload

2013-09-06 | GSE50638 | GEO

Transcription profiling of rat 3 months old male Wistar animals by aorto-caval fistula reveals alterations in systemic substrate metabolism in rat volume-overload model of chronic heart failure

Project description:Abnormalities in metabolism of energetic substrates may play a role in progression of chronic heart failure (HF). The goal of the study was to examine the extent and mechanisms of metabolic alterations in rat model of chronic HF due to volume overload. Volume overload was induced in 3 months old male Wistar rats by aorto-caval fistula. In the phase of symptomatic HF (after 21 weeks), we performed myocardial gene expression analysis. Cardiac tissue gene expression analysis showed downregulation of enzymes of respiratory cycle, mitochondrial fatty acid (FA) oxidation and attenuated expression of proteins responsible for FA translocation/transport (CD36/FAT, FABP3, FATP-1). Simultaneously, we performed gene expression analysis of fat tissue.

2011-10-05 | E-MTAB-190 | biostudies-arrayexpress

expression data from Zbtb17 cKO mouse heart after pressure overload

Project description:whole gene expression analysis for ZBTB17 cKO mice after transverse aortic constriction (TAC) We used microarrays to detail the global programme of gene expression underlying hypertrophy and heart failure process upon pressure overload 16 male mice, 4 mice per group, were selected at 8-10 weeks old for 4 weeks of TAC and SHAM and collected samples for RNA extraction and hybridization on Affymetrix microarrays. We included corresponding controls.

2013-09-06 | E-GEOD-50638 | biostudies-arrayexpress

Pressure overload greatly promotes neonatal right ventricular cardiomyocyte proliferation - a new model for heart regeneration study

Project description:Background: Current mammalian model for heart regeneration research is limited in apex amputation or myocardium infarction, both of which are controversy. Moreover, RNAseq demonstrated there were a very limited set of differential expressed genes between sham and operation heart in the myocardium infarction model. Here we investigated whether pressure overload in the right ventricle(RV), a common phenomenon in congenital heart disease children, could be a better animal model for heart regeneration study when consider cardiomyocyte(CM) proliferation as the most important index. Methods and results: Pressure overload was induced by pulmonary artery banding (PAB) on day1 and confirmed by echocardiography and hemodynamic measurements at postnatal day 7(P7). RNAseq analyses of purified RVCM at P7 from PAB and sham-operated rats revealed there were 5469 differential expressed genes between these two groups. GO and KEGG analysis showed that these genes mainly mediated mitosis and cell division. Cell proliferation assay indicates a continuous over-proliferation of RVCM after PAB, in particular for P3. In addition, there were ~2 times-fold increase of Ki67/Phh3 -positive CM in human overload RV compared to non-overload RV. Other features about this model included CM hypotrophy and no fibrosis.. Conclusions: Pressure overload profoundly promotes RVCM proliferation in the neonatal stage both in rats and human beings, activated a regeneration-specific gene program, and may offer a better alternative animal model for heart regeneration research..

2020-08-19 | GSE139561 | GEO

Differential gene expressions in the hypertensive heart using pressure-overload model

Project description:Differential gene expressions were constructed through expression profiling of a total of 26,423 genes in the hypertrophic heart followed by pressure-overload model, then 537 significant differential genes were filtered based on the criteria (p<0.05 and fold change >1.5).

2016-11-04 | GSE89493 | GEO

Identification of differentially expressed genes in mice with chronic heart failure induced by pressure overload

Project description:To investigate the mechanism of chronic heart failure induced by pressure overload, we established an animal model in mice by transverse aortic constriction (TAC), with mice undergoing sham surgery as control.

2023-10-31 | GSE228199 | GEO

Skeletal muscle derived Musclin protects the heart during pathological overload

Project description:Skeletal muscle derived Musclin protects the heart during pathological overload

| PRJNA530525 | ENA