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ATAC-Sequencing of human fetal PreProB and ProB progenitors.

ABSTRACT: ATAC-Sequencing of human fetal PreProB and ProB progenitors.

PROVIDER: PRJNA507171 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8247850_1.fastq.gz	Fastqsanger.gz
	SRR8247850_2.fastq.gz	Fastqsanger.gz
	SRR8247851_1.fastq.gz	Fastqsanger.gz
	SRR8247851_2.fastq.gz	Fastqsanger.gz
	SRR8247852_1.fastq.gz	Fastqsanger.gz

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ATAC-Sequencing of human fetal PreProB and ProB progenitors.

Project description:Human lymphopoiesis is a dynamic life-long process that starts in utero at 6 weeks gestation; however developmental pathways defining fetal lymphopoiesis are largely unexplored. While the first committed B-progenitor in normal human bone marrow (BM) is a CD10+ve ProB-progenitor, the identification of a CD10-ve B-progenitor (Pre/ProB progenitor) in cord blood suggests there may be a second B-lymphoid development program in fetal life. Here, we provide a comprehensive analysis of the fetal B-cell developmental hierarchy and report the presence of both PreProB- and ProB-progenitors in fetal tissues and describe their ontogeny, molecular and functional characteristics. PreProB- and ProB-progenitors appear early in the first trimester in fetal liver, followed by a sustained second wave of B-progenitor development in fetal BM, where together they form >40% of the total HSC/progenitor pool. Unexpectedly, one-third of the B-progenitors (13±1% of lin-CD34+ cells) are CD10-ve PreProB-progenitors while, by contrast, PreProB-progenitors are virtually undetectable in adult BM. Single-cell transcriptomics and functional assays place PreProB- upstream of ProB-progenitors, identifying them as the first B-lymphoid restricted progenitor in human fetal life. Fetal BM PreProB- and ProB-progenitors both give rise solely to B-lineage cells yet they are transcriptionally distinct. PreProB- unlike ProB-progenitors, continue to express a number of key myeloid and stem cell genes and share some transcriptomic signatures with CD10-ve B-progenitor infant acute lymphoblastic leukemia blast cells. Our data identify PreProB-progenitors as the earliest B-lymphoid restricted progenitor in human fetal life, and suggest that this fetal-restricted committed B-progenitor might provide a permissive cellular context for prenatal B-progenitor leukemia initiation.

2019-08-06 | GSE122989 | GEO

Bulk (100cell) RNA-Sequencing of human fetal haematopoietic stem, progenitor and B cells

2019-08-06 | GSE122982 | GEO

RNA- and ATAC-seq data of Wt, Ebf1-KO and Pax5-KO FL-ProB-cells.

Project description:EBF1 and PAX5 are crucial factors for B-cell development. Knock down of either factor leads to an arrest in differentiation. RNA-seq data of WT, Ebf1-/- and Pax5-/- FL-ProB cells allowed for identification of activated and repressed target genes. ATAC-seq data of corresponding cell populations could connect gene activity and chromatin accessibility. This finding provided an explanation to the lineage instability observed in early B-cell progenitors. Taken together, the reported data provide an increased insight into mechanisms governing regulation of stage- and lineage specific transcription in early B-lymphocyte development.

2018-06-08 | GSE92434 | GEO

The role of Erg in B cell development (ATAC-Seq)

Project description:ATAC-seq of pre-proB, proB and preB populations from Ergfl/fl bone marrow, pre-proB cell population from Rag1CreT/+;ErgΔ/Δ bone marrow, and pre-proB, proB and preB populations from from Rag1CreT/+;ErgΔ/Δ;IgHVH10tar bone marrow.

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ATAC-seq data of miR-195 transduced Ebf1-KO FL-ProB-cells.

Project description:ATAC-seq data of miR-195 transduced Ebf1-KO FL-ProB-cells.

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Quantitative secretome analysis of fetal and adult hematopoietic progenitors during leukemia initiation and its response to Fibulin and/or Fibronectin treatment.

Project description:We describe the proteomic composition of the secretome of fetal and adult hematopoietic progenitors during MLL-rearranged (MLLr) leukemia initiation as well as the pre-leukemic cells’ secretion response to the treatment with Fibulin (Fbln1) and/or Fibronectin (Fn1) using data-independent acquisition mass spectrometry analysis.

2024-04-02 | PXD049014 | Pride

ATAC-seq of dendritic cell progenitors

Project description:ATAC-seq of dendritic cell progenitors

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ATAC-Seq of foetal blood progenitors

Project description:ATAC-Seq of foetal blood progenitors

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Single-cell sequencing of yolk sac and fetal liver hematopoietic progenitors

Project description:Erythro-myeloid progenitors (EMP) and their progeny were labeled with YFP in mouse embryos using genetic fate mapping (constitutive Csf1r-iCre;Rosa26-eYFP or inducible Csf1r-MeriCreMer;Rosa26-eYFP injected at embryonic day E8.5 with 4-hydroxytamoxifen). EMP-derived progenitors (Lin- Kit+ YFP+) were sorted by FACS for single-cell transcriptomic analysis using the MARS-Seq approach. The purpose was to uncover heterogeneity and differentiation trajectories of EMP by comparing their transcriptional status at different developmental timepoints (E9.5, E10.5 and E12.5) and across niches (yolk sac and fetal liver).

2021-02-05 | GSE166223 | GEO

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