Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus Gata2 +9.5 Ets C>T transition untreated and 10 days post 5-FU LSK cells

ABSTRACT: Mus musculus Gata2 +9.5 Ets C>T transition untreated and 10 days post 5-FU LSK cells

PROVIDER: PRJNA507449 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8258120.fastq.gz	Fastqsanger.gz
	SRR8258140.fastq.gz	Fastqsanger.gz
	SRR8258111.fastq.gz	Fastqsanger.gz
	SRR8258121.fastq.gz	Fastqsanger.gz
	SRR8258131.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Mus musculus Gata2 +9.5 Ets C>T transition untreated and 10 days post 5-FU LSK cells

Project description:The development and function of stem and progenitor cells that produce blood cells are vital in physiology. GATA2 mutations cause immunodeficiency, myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). GATA-2 physiological activities necessitate that it be strictly regulated and cell type-specific enhancers fulfill this role. The +9.5 intronic enhancer harbors multiple conserved cis-elements, and germline mutations of these cis-elements are pathogenic in humans. Since mechanisms underlying how GATA2 enhancer disease mutations impact hematopoiesis and pathology are unclear, we generated mouse models of the enhancer mutations. While a multi-motif mutant was embryonic lethal, a single-nucleotide Ets motif mutant was viable and steady-state hematopoiesis was normal. However, the Ets motif mutation abrogated stem/progenitor cell regeneration following stress. These results reveal a new mechanism in human genetics in which a disease mutation inactivates enhancer regenerative activity, while sparing developmental activity. Mutational sensitization to stress that instigates hematopoietic failure constitutes a paradigm for GATA-2-dependent pathogenesis.

2019-02-06 | GSE123080 | GEO

Gene expression changes in embryonic PECAM-1+ cells from Gata2 +9.5 enhancer targeted mice

Project description:We have generated a nonconditionall deletion of an E-box - GATA motif composite element from a putative enhancer 9.5 kb downstream of the Gata2 1S promoter. The mutation results in lethality at E13.5 to E14.5 with embryos exhibiting dectects in definitive hematopoiesis and vasculature integrity. Hematopoietic stem and progenitor cells are nearly absent from these mice and Gata2 expression is severely reduced in Pecam-1+ endothelial cells from E12.5-12.5 embryos but is expressed at normal levels in the embryonic brain. We have employed gene expression profiling in Pecam-1+ cells from wild type and Gata 2+9.5 mutant littermates to identify genes involved in maintaining vascular integrity. The Gata2+9.5 mutation was backcrossed into the C57Bl/6J background. Anti-CD31 antibody bound to magnetic beads was used to enrich for PECAM-1+ cells from four Gata2+9.5 E12.5 embryos and four wild type littermates from two litters. A single RNA sample from each embryo was used for array hybridization.

2012-07-16 | E-GEOD-39300 | biostudies-arrayexpress

Gene expression changes in embryonic PECAM-1+ cells from Gata2 +9.5 enhancer targeted mice

2012-07-17 | GSE39300 | GEO

Next Generation Sequencing of Wild Type and Gata2+/- HSCs

Project description:Gata2, a zinc finger TF, is essential for the generation and survival of HSCs in the embryo and has been implicated in the pathogenesis of AML, yet the requirement for Gata2 in adult HSCs and LSCs remains unclear. Using a conditional mouse model where Gata2 was deleted specifically in hematopoietic cells, we show that knockout of Gata2 leads to a rapid and complete cell-autonomous loss of adult HSCs. We then performed RNA-seq in sorted HSCs (LSK CD48- CD150+) from control and Gata2+/fl;Vav-iCre+ 8-to-10-week old mice.

2019-06-25 | GSE133248 | GEO

FU methane

Project description:FU methane Metagenome

| PRJNA756936 | ENA

Populus trichocarpa strain:HR.II.9.5 | cultivar:HR.II.9.5

Project description:Populus trichocarpa HR.II.9.5 Resequencing genome sequencing

| PRJNA332146 | ENA

Expression data from WT and GABPalpha-deficient Flt3(-) LSK cells

Project description:GABPalpha is an Ets family transcription factor and involved in regulation of both basic cellular functions such as cell cycle progression and tissue-specific biological processes. We found that GABPalpha is critically required for survival and differentiation of hematopoietic stem cells. We used microarrays to detect gene expression changes in Flt3(-) LSK cells (which contains both long-term and short-term hematopoietic stem cells) by GABPalpha deficiency.

2011-07-28 | GSE23341 | GEO

GATA2 Deficiency

Project description:GATA2 Deficiency

| PRJNA684644 | ENA

GATA2 Deficiency

Project description:GATA2 Deficiency

| PRJNA684645 | ENA

FU methane winter

Project description:FU methane winter Metagenome

| PRJNA797915 | ENA