Biotic stress-induced priming and de-priming of transcriptional memory in Arabidopsis
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PROVIDER: PRJNA507954 | ENA |
REPOSITORIES: ENA
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Project description:Biotic stress-induced priming and de-priming of transcriptional memory in apple.
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Project description:In order to identify primed (and memorized) genes resulting from BTH and/ or flg22 application, we performed a series of microarray-based transcription profiles. 7 days after germination Arabidopsis plants were treated the first time with the indicated treatment and second time 10 days after germination with the indicated treatment. Plants were harvested and RNA was extracted 21 days after germination. BTH was applied as formulated compound Bion. The sample name reflects the treatment order. Samples with the name water_water are control treatments which were treated 7 and 10 days after germination with water. The sample with the name Bion_flg22 was treated 7 days after germination with BTH and 10 days after germination flg22.
2018-12-04 | GSE123073 | GEO
Project description:Under natural growth conditions, plants experience various and repetitive biotic and abiotic stresses. Salicylic acid (SA) is a key phytohormone involved in the response to biotic challenges. Application of synthetic SA analogues can efficiently prime defense responses, and leads to improved pathogen resistance. Because SA analogues can result in long-term priming and memory, we identified genes for which expression was affected by the SA analogue and explored the role of DNA methylation in this memorization process. We show that treatments with an SA analogue can lead to long-term transcriptional memory of particular genes in Arabidopsis. We found that subsequent challenging of such plants with a bacterial elicitor reverted this transcriptional memory, bringing their expression back to the original pre-treatment level. We also made very similar observations in apple (Malus domestica), suggesting that this expression pattern is highly conserved in plants. Finally, we found a potential role for DNA methylation in the observed transcriptional memory behavior. We show that plants defective in DNA methylation pathways displayed a different memory behavior. Our work improves our understanding of the role of transcriptional memory in priming, and has important implication concerning the application of SA analogues in agricultural settings.
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Project description:In order to identify primed (and memorized) genes resulting from BTH and/ or flg22 application, we performed a series of microarray-based transcription profiles. 14 days after propagation plants were treated the first time with the indicated treatment and second time 17 days after propagation with the indicated treatment. Plants were harvested and RNA was extracted 31 days after propagation. BTH was applied as formulated compound Bion. The sample name reflects the treatment order. Samples with the name water_water are control treatments which were treated 14 and 17 days after propaghation with water. The sample with the name Bion_flg22 was treated 14 days after germination with BTH and 17 days after germination flg22.
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Project description:Plants from temperate regions can be primed by exposure to low, non-freezing temperatures resulting in improved freezing tolerance. Whereas the molecular and metabolic basis of cold priming has been investigated in detail, hardly anything is known about memory of a previous cold event under warm conditions and a following low temperature triggering event. We show that three days of cold priming at 4°C, a seven-day lag phase at 20°C and a triggering treatment of 4°C improved the freezing tolerance of Arabidopsis Col-0 and other accessions compared to plants that were not primed before. Transcripts, metabolites and lipids as possible molecular determinants of this increase in freezing tolerance were investigated in Arabidopsis accessions Col-0 and N14 after priming, memory phase and triggering by Illumina-based RNA-Seq, GC-MS metabolite profiling and UPLC FT-MS-based lipidomics. Comparing primed and triggered with only triggered samples 93 and 128 unique differentially expressed genes could be identified in Col-0 and N14, together with three and six significantly changed lipids and one metabolite in N14. Possible functions of these candidates will be discussed. This work identified for the first time molecular and metabolic changes accompanying cold stress memory and triggering by a second cold stress.
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Project description:TNIK signaling imprints CD8+ T memory formation early after priming
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Project description:A role of chromatin in plant memory of environmental stress has been proposed. In this study ChIP-Seq was employed to investigate whether a transient mild salt treatment of Arabidopsis thaliana plant at seedling stage (M-^Qpriming treatmentM-^R: 50 mM NaCl for 24 hours) altered genome-wide profiles of four histone modifications; H3K4me2, H3K4me3, H3K9me2 and H3K27me3. Comparison of histone modification profiles between primed and non-primed plants harvested immediately after the priming treatment showed differences in all histone modifications but were strongest and most abundant for H3K27me3. We subsequently explored whether these changes were maintained over an extensive period of growth in control conditions. Additional ChIP-Seq analysis showed that differences in the H3K27me3 profiles between primed and non-primed plants were still apparent after a growth period of ten days in control conditions. The results provide first indication for a potential role of H3K27me3 in long-term somatic memory of abiotic stress events in plants. The obtained dataset was compared with RNA-seq data measured in the same plant material (see parallel submission of RNA-Seq data in ArrayExpress: https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1668).
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