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Randomized PAM depletion of AsCas12a

ABSTRACT: Randomized PAM depletion of AsCas12a

PROVIDER: PRJNA508763 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8286606.fastq.gz	Fastqsanger.gz
	SRR8286607.fastq.gz	Fastqsanger.gz

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Randomized PAM depletion of AsCas12a

Project description:We report the PAMs of AsCas12a using a cell-free TXTL-based cleavage assay. By adding randomized PAM library and AsCas12a-gRNA in vitro, functional PAM sequences were cleaved, while non-functional PAMs remained. By amplifying the non-cleaved DNA, we use next-generation sequencing to analyze the depletion of functional PAMs of AsCas12a.

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