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Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell

ABSTRACT: Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell

PROVIDER: PRJNA508975 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7611045.fastq.gz	Fastqsanger.gz
	SRR7611046.fastq.gz	Fastqsanger.gz
	SRR7611047.fastq.gz	Fastqsanger.gz
	SRR7611048.fastq.gz	Fastqsanger.gz
	SRR8308730_1.fastq.gz	Fastqsanger.gz

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Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell [part 1]

Project description:The weekly turnover of the intestinal epithelium is driven by multipotent, Lgr5+, crypt base columnar cells (CBCs). In response to injury, however, Lgr5+ cells are lost but then re-emerge and are required for successful recovery. How these resurgent Lgr5+ stem cells arise is unclear. We transcriptionally profiled single cells from regenerating intestinal epithelia and identified a unique cell type we term the revival stem cell (rSC). rSCs are mutually exclusive to CBCs and are distinguished by elevated expression of cell survival and DNA repair genes. In homeostasis, rSCs are extremely rare, but nevertheless give rise to all the major cell types of the intestine including crypt-villus axes. After damage rSCs display a 20-fold, Yap-dependent, transient expansion, reconstitute the Lgr5+ CBC compartment and are required to regenerate a functional intestine. These studies define a unique stem cell phenotype that is mobilized by damage to reconstitute the intestinal epithelium.

2019-02-28 | GSE117783 | GEO

Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell [part 2]

2019-02-28 | GSE123515 | GEO

Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell [part 1]

Project description:Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell [part 1]

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Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell [part 2]

Project description:Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell [part 2]

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Single-Cell Transcriptomes of the Regenerating Intestine Reveal a Revival Stem Cell

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-02-28 | GSE123516 | GEO

Gene expression profile of regenerating intestine in the sea cucumber H. glaberrima

2009-05-21 | GSE16182 | GEO

Gene expression profile of regenerating intestine in the sea cucumber H. glaberrima

Project description:Regenerating intestine tissues were compared with normal (non-regenerating tissues) to determine the gene expression profile at early stages of regeneration (3, 7 and 14 days post- evisceration (dpe)). Immuno activated tissues with LPS were compared with Normal (non-regenerating tissues) and regenerating tissues (3 and 7 dpe) to determine the gene expression of immune related genes in the sea cucumber and it's expression during early stages of regeneration. 16 Total arrays. 8 comparisons with 8 dye swap replicates. Normal Vs. (3, 7 and 14 days of regeneration), and Vs. (LPS) 2 microarray slides used. Each slide with 8 Arrays (8 x 15k). 15,744 probes printed per array. (all 16 arrays are identically printed). 7,209 ESTs from Normal, 3 dpe and 7 dpe tissues were used to design the probes. Two probes per EST from diferent regions of the sequence (as a technical replicate). 536 technical controls and more than 600 internal biological controls (from different species).

2010-05-18 | E-GEOD-16182 | biostudies-arrayexpress

p53 promotes revival stem cells in the regenerating intestine after severe radiation injury.

Project description:Ionizing radiation induces cell death in the gastrointestinal (GI) epithelium by activating p53. However, p53 also prevents animal lethality caused by radiation-induced acute GI syndrome. Through single-cell RNA-sequencing of the irradiated mouse small intestine, we find that p53 target genes are specifically enriched in regenerating epithelial cells that undergo fetal-like reversion, including revival stem cells (revSCs) that promote animal survival after severe damage of the GI tract. Accordingly, in mice with p53 deleted specifically in the GI epithelium, ionizing radiation fails to induce fetal-like revSCs. Using intestinal organoids, we show that transient p53 expression is required for the induction of revival stem cells and is controlled by an Mdm2-mediated negative feedback loop. Together, our findings reveal that p53 suppresses severe radiation-induced GI injury by promoting fetal-like reprogramming of irradiated intestinal epithelial cells.

| S-EPMC11001929 | biostudies-literature

Phosphoproteome Dynamics Mediate Revival of Bacterial Spores

Project description:Background: Bacterial spores can remain dormant for decades, yet harbor the exceptional capacity to rapidly resume metabolic activity and recommence life. Although germinants and their corresponding receptors have been known for more than 30 years, the molecular events underlying this remarkable cellular transition from dormancy to full metabolic activity are only partially defined. Results: Here, we examined whether protein phospho-modifications occur during germination, the first step of exiting dormancy, thereby facilitating spore revival. Utilizing Bacillus subtilis as a model organism, we performed phosphoproteomic analysis to define the Ser/Thr/Tyr phosphoproteome of a reviving spore. The phosphoproteome was found to chiefly comprise newly identified phosphorylation sites located within proteins involved in basic biological functions, such as transcription, translation, carbon metabolism and spore-specific determinants. Quantitative comparison of dormant and germinating spore phosphoproteomes revealed phosphorylation dynamics, indicating that phospho-modifications could modulate protein activity during this cellular transition. Furthermore, by mutating select phosphorylation sites located within proteins representative of key biological processes, we established a functional connection between phosphorylation and the progression of spore revival. Conclusions: In this study we provide for the first time a phosphoproteomic view of a germinating bacterial spore. We show that the spore phosphoproteome is dynamic and present evidence that phosphorylation events play an integral role in facilitating spore revival.

2015-08-27 | PXD002559 | Pride

Project description:p53 promotes revival stem cells in the regenerating intestine after severe radiation injury

| PRJNA910548 | ENA