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Salmo salar

ABSTRACT: Genomic and transcriptomic analysis of amoebic gill disease resistance in Atlantic salmon

PROVIDER: PRJNA509327 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8304480_1.fastq.gz	Fastqsanger.gz
	SRR8304480_2.fastq.gz	Fastqsanger.gz
	SRR8304490_1.fastq.gz	Fastqsanger.gz
	SRR8304490_2.fastq.gz	Fastqsanger.gz
	SRR8304500_1.fastq.gz	Fastqsanger.gz

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Salmo salar

Project description:Atlantic Salmon Amoebic Gill Disease Study

| PRJNA94083 | ENA

Investigation of the host response of naïve Atlantic salmon (Salmo salar) inoculated with Paramoeba perurans prior to the onset of clinical symptoms

Project description:Amoebic Gill Disease (AGD), caused by the ectoparasite Paramoeba perurans (P. perurans) is characterised by hyperplasia of the gill epithelium and lamellar fusion and has become recognised as one of the most significant health threats in salmon farming . In this study, the gill and serum proteomes of Atlantic salmon inoculated with P. perurans, across multiple timepoints post-challenge, were analysed. The expression of proteins with established roles in innate immunity, across various timepoints, was compared with expression in naïve Atlantic salmon to elucidate the host response to gill colonisation.

2021-04-16 | PXD022101 | Pride

Global gene expresssion profiling the gills of amoebic gill disease (AGD) affected Atlantic salmon (Salmo salar)

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Host-pathogen interaction of Atlantic salmon (Salmo salar) and the ectoparasite, Neoparamoeba perurans in amoebic gill disease

Project description:Marine farmed Atlantic salmon (Salmo salar) are repeatedly susceptible to amoebic gill disease (AGD) caused by the ectoparasite Neoparamoeba perurans over their life cycle. The parasite elicits a highly localized response within the gill epithelium mucosa resulting in multifocal mucoid patches at the site of parasite attachment. This host-pathogen response drives a complex immune reaction within the pathology of the disease, which remains poorly understood. A dual RNA-seq approach was employed using Illumina sequencing technology to investigate both the linteraction between the host and the parasite.

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single nuclei RNAseq data from Atlantic salmon (Salmo salar) gill

Project description:An snRNAseq analysis of the Atlantic salmon gill to characterise its cellular complexity and development.

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Salmo salar

Project description:Global gene expresssion profiling the gills of amoebic gill disease (AGD) affected Atlantic salmon (Salmo salar)

| PRJNA103439 | ENA

Transcription profiling of gill, liver and kidney from amoebic gill disease infected and disease-free Atlantic salmon

Project description:The transcriptome response of 12 amoebic gill disease (AGD) affected Atlantic salmon were compared to 6 AGD naive Atlantic salmon at 19 days post infection. The transcriptome response was examined in the gill, liver and anterior kidney.

2008-09-01 | E-MEXP-1286 | biostudies-arrayexpress

Transcription profiling of gill from ameobic gill disease resistant and susceptible Atlantic salmon

Project description:We compared gill transcriptomes of two groups of Atlantic salmon, one designated putatively resistant, and one designated susceptible to amoebic gill disease (AGD).

2008-12-01 | E-MEXP-1396 | biostudies-arrayexpress

Global gene expresssion profiling the gills of amoebic gill disease (AGD) affected Atlantic salmon (Salmo salar)

Project description:Amoebic gill disease (AGD) is an ectoparasitic condition of some farm-reared marine fish and is caused by Neoparamoeba perurans. Tanks housing Atlantic salmon (Salmo salar) were inoculated with Neoparamoeba perurans and fish were sampled at 36 days postinoculation (pi.). AGD-affected gill tissue was dissected from N. perurans infected fish, and a DNA microarray was used to compare global gene expression against tissues from AGD-naive fish. To determine whether the changes in gene expression were restricted to AGD-lesions, lesion tissue from AGD-affected fish was also compared with non-lesion gill tissue dissected from the same gill arch. Samples were assessed using a DNA microarray. mRNA from lesion and non-lesion gill tissue was amplified and labeled. Six biological and two technical replicates were utilised to hybridise to 12 arrays using amplified RNA from AGD-affected lesion gill tissue with AGD-naive fish as a control. Four biological and two technical replicates were utilised to hybridise to 8 arrays using amplified RNA from AGD-affected lesion gill tissue with non-lesion tissue from the same gill arch as a control. The assignment of microarrays to treatment groups for hybridization was randomised by using a random number generator.

2010-05-25 | E-GEOD-9595 | biostudies-arrayexpress

Gill and liver transcript expression changes associated with gill damage in Atlantic salmon (Salmo salar)

Project description:Gills of teleost fish represent a vital multifunctional organ; however, they are subjected to environmental stressors, causing gill damage. Gill damage is associated with significant losses in the Atlantic salmon aquaculture industry. Gill disorders due to environmental stressors are exacerbated by global environmental changes, especially with open-net pen aquaculture (as farmed fish lack the ability to escape those events). The local and systemic response to gill damage, concurrent with several environmental insults, are not well investigated. We performed field sampling to collect gill and liver tissue after several environmental insults. Using a 44K salmonid microarray platform, we aimed to compare the transcriptomes of pristine and moderately damaged gill tissue. The gill damage-associated biomarker genes and associated qPCR assays arising from this study will be valuable in future research aimed at developing therapeutic diets to improve farmed salmon gill health.

2022-03-24 | GSE186728 | GEO

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