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Endothelial Sash1 is required for alveolar surfactant production through nitric oxide signaling

ABSTRACT: Endothelial Sash1 is required for alveolar surfactant production through nitric oxide signaling

PROVIDER: PRJNA510121 | ENA |

REPOSITORIES: ENA

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Endothelial Sash1 is required for alveolar surfactant production through nitric oxide signaling

Project description:Sash1 acts as a scaffold in TLR4 signaling. We generated Sash1-/- mice, which die in the perinatal period due to respiratory distress. Constitutive or endothelial-restricted Sash1 loss leads to a reduction of surfactant-associated protein synthesis. We show that Sash1 interacts with β-arrestin 1 downstream of the TLR4 pathway to activate Akt and eNOS in microvascular endothelial cells. Generation of nitric oxide downstream of Sash1 in endothelial cells activated cGMP in adjacent alveolar type 2 cells to induce transcription of surfactant genes. Thus we identify a critical cell nonautonomous function for Sash1 in embryonic development in which endothelial Sash1 affects alveolar type 2 cells and promotes pulmonary surfactant production through nitric oxide signaling. Lack of pulmonary surfactant is a major cause of respiratory distress and mortality in preterm infants, and these findings identify the endothelium as a potential target for therapy.

2019-05-01 | GSE123889 | GEO

Role of nitric oxide and nitric oxide synthase isoforms in pulmonary emphysema.

Project description:The purpose of this study is to comprehensively elucidate the role of nitric oxide and nitric oxide synthase isoforms in pulmonary emphysema using cap analysis of gene expression (CAGE) sequencing.

2021-01-18 | GSE164521 | GEO

Oleic acid-dependent modulation of Nitric Oxide Associated 1 protein levels regulate nitric oxide mediate signaling in plant defense.

2012-08-01 | GSE36797 | GEO

Oleic acid-dependent modulation of Nitric Oxide Associated 1 protein levels regulate nitric oxide mediate signaling in plant defense.

Project description:Oleic acid-dependent modulation of Nitric Oxide Associated 1 protein levels regulate nitric oxide- mediate signaling in plant defense. Nitric Oxide, Oleic acid, Salicylic acid, Arabidopsis, Defense Wild-type Col-0, mutant genotypes ssi2, ssi2 act1 and ssi2 sid2 with three biological replicates were analyzed (one array each)

2012-07-31 | E-GEOD-36797 | biostudies-arrayexpress

Role of nitric oxide and nitric oxide synthase isoforms in pulmonary emphysema.

Project description:Role of nitric oxide and nitric oxide synthase isoforms in pulmonary emphysema.

| PRJNA691047 | ENA

Transcriptome response to nitric oxide in Pseudomonas aeruginosa

Project description:DNA microarray analysis was employed to investigate the transcriptome response to nitric oxide in Pseudomonas aeruginosa. We focused on the role played by the nitric oxide-response regulators DNR and FhpR and an oxygen-response regulator ANR in the response. The transcriptome profiles of the P. aeruginosa strains before and after exposure to nitric oxide under the microaerobic conditions were analyzed. Wild type, its anr, dnr, and fhpR mutants, and the anr mutant that express dnr were used for the analyses. Pseudomonas aeruginosa wild type (PAO1ut), anr mutant (RManr), dnr mutant (RMdnr), anr mutant that constitutively expresses DNR (RManrEXdnr), and fhpR mutant (PDM2665) were cultivated microaerobically in LB in 1-liter jar fermenter. When optical density at 600 nm reached 0.3, nitric oxide-saturated water was added to the medium (final nitric oxide concentration was 20 micro-M). RNA was isolated from a 10 ml aliquot of the culture prior to the addition of nitric oxide and at 5 min after the addition. The experiment was performed in duplicate independent cultures.

2014-12-08 | E-GEOD-58862 | biostudies-arrayexpress

Transcriptome response to nitric oxide in Pseudomonas aeruginosa

2014-12-08 | GSE58862 | GEO

Nitric Oxide-Releasing Acetylsalicyclic Acid in Preventing Colorectal Cancer in Patients at High Risk of Colorectal Cancer

Project description:RATIONALE: Chemoprevention is the use of certain drugs to keep cancer from forming, growing, or coming back. The use of nitric oxide-releasing acetylsalicyclic acid may prevent colorectal cancer. PURPOSE: This randomized phase I trial is studying the side effects and best dose of nitric oxide-releasing acetylsalicyclic acid in preventing colorectal cancer in patients at high risk of colorectal cancer.

| 2029365 | ecrin-mdr-crc

Testing the effects of nitric oxide (oxidative) stress on Caulobacter transcription

Project description:The goal of this study was to measure the effects of nitric oxide exposure (using DETA NONOate as a nitric oxide donor) on transcription in Caulobacter.

2010-06-21 | GSE21139 | GEO

Gene expression in response to exposure to nitric oxide

Project description:To analyze gene expression pattern in response to nitric oxde in Saccharomyces cerevisiae, we performed microarray analysis using yeast cells treated with a nitric oxide donor S-nitroso-N-acetyl-DL-penicillamine (SNAP). S. cerevisiae S288c strain was cultured in synthetic dextrose (SD) minimal medium up to log phase (OD600 = 1), and then treated with 100 micro molar of SNAP for 10, 30, or 60 min. After treatment with SNAP, cells were disrupted adn total RNA was extracted. We commissioned following experiments to BIO MATRIX RESEARCH Inc. Nitric oxide-induced gene expression in S. cerevisiae was measured at 10, 30, or 60 minutes after exposure to 100 micro molar of nitric oxide donor SNAP.

2014-09-30 | E-GEOD-61836 | biostudies-arrayexpress

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