Project description:The genes induced by mechanical stimuli may be also involved in disease resistance and wood formation and development in Acacia koa. If so, mechanically stressed A. koa may be used as a model to study disease resistance and wood formation and development. Microarray analysis was performed to determine expression levels of 4,000 genes related to disease resistance and wood development in Acacia koa in response to mechanical stimuli (touch).
Project description:Nectaries are the glands responsible for nectar secretion. In the tree Acacia cornigera, extrafloral nectaries produce nectar primarily consumed by mutualistic ant colonies. To understand the genetic programming underlying nectar production, and in particular the connection of the jasmonic acid pathway with the mutualistic ants, extrafloral nectaries tissue was collected at ten time points and conditions, with RNA being isolated and subjected to Illumina RNA-seq analysis.
Project description:The genes induced by mechanical stimuli may be also involved in disease resistance and wood formation and development in Acacia koa. If so, mechanically stressed A. koa may be used as a model to study disease resistance and wood formation and development. Microarray analysis was performed to determine expression levels of 4,000 genes related to disease resistance and wood development in Acacia koa in response to mechanical stimuli (touch). RNA was extracted from two groups of A. koa seedlings, (1) mechanically stressed and (2) unstressed koa seedlings. Each group had two biological replicates (n=2), where n represents pools of approcimately 20 individuals.
