Project description:Transcriptome profile of Arabidopsis wild-type (Col-0) and mutants of the plastidic retrograde signalling pathway (gun1-1,gun4-1, gun5-1) germinated in the presence of 5µM Norflurazon for 5d in continious light (100µE). Analysis was performed on biological triplicates of total leaf RNA using paired-end Illumina Hi/MiSeq technology.
2019-10-18 | GSE104868 | GEO
Project description:Illumina NextSeq 500 paired end sequencing
Project description:Genome editing was conducted on a t(3;8) K562 model to investigate the effects of deleting different modules or CTCF binding sites within the MYC super-enhancer. To check mutations after targeting with CRISPR-Cas9 we performed amplicon sequencing using the Illumina PCR-based custom amplicon sequencing method using the TruSeq Custom Amplicon index kit (Illumina). The first PCR was performed using Q5 polymerase (NEB), the second nested PCR with KAPA HiFi HotStart Ready mix (Roche). Samples were sequenced paired-end (2x 250bp) on a MiSeq (Illumina).