Project description:Macrophages are at the front line of the innate immune system and differentiate from monocytes after infiltrating the infected tissue. Metabolic pathways are central to macrophage biology. We studied transcriptional differences between Rapamycin treated in vitro activated macrophages and untreated peripheral blood derived in vitro actiavted macrophages. We used microarrays to detail the global programme of gene expression in Rapamycin treated adult blood derived M(IL-10) and M(IFN-γ) macrophages and untreated adult blood derived M(IL-10) and M(IFN-γ)macropahges
Project description:In order to study the gene expression profiles of monocyte and macrophages, we collected three type of cells and performed pair-wised comparison. It includes human peripheral blood monocyte (MONO), human peripheral blood monocyte derived macrophages treated with M-CSF (MACRO)and primary alveolar macrophages (BAL). All the experiments are performed comparing two of the three cell types from the same person (total 4 persons). Totally we got three set of microarray data, MONO/MACRO, MONO/BAL and MACRO/BAL with 4 biological replicates.
Project description:The risk of sepsis is particularly high in neonates compared to older children and adults; however the reasons behind are incompletely understood. Macrophages are at the front line of the innate immune system and differentiate from monocytes after infiltrating the infected tissue. We studied transcriptional differences between cord blood-derived in vitro activated macrophages and adult peripheral blood derived in vitro actiavted macrophages. We used microarrays to detail the global programme of gene expression in cord blood derived M(IL-10) and M(IFN-γ) and in adult blood derived M(IL-10) and M(IFN-γ)macropahges
Project description:We investigate the impact of an initial stimulation and a re-stimulation in altering transcriptional responses in both peripheral blood-monocyte derived macrophages and human pluripotent stem cell derived macrophages.